Institute of Functional Nano & Soft Materials (FUNSOM), Jiangsu Key Laboratory for Carbon-based Functional Materials and Devices, Soochow University, Suzhou, Jiangsu, 215123, China.
Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215123, China.
Adv Healthc Mater. 2024 Mar;13(8):e2303017. doi: 10.1002/adhm.202303017. Epub 2024 Feb 11.
How to promote wound healing is still a major challenge in the healthcare while macrophages are a critical component of the healing process. Compared to various bioactive drugs, many plants have been reported to facilitate the wound healing process by regulating the immune response of wounds. In this work, a Three-dimensional (3D) printed hydrogel scaffold loaded with natural Centella asiatica extract (CA extract) is developed for wound healing. This CA@3D scaffold uses gelatin (Gel) and sodium alginate (SA) with CA extract as bio-ink for 3D printing. The CA extract contains a variety of bioactive compounds that make the various active ingredients in Centella asiatica work in concert. The printed CA@3D scaffold can fit the shape of wound, orchestrate the macrophages and immune responses within the wound, and promote wound healing compared to commercial wound dressings. The underlying mechanism of promoting wound healing is also illuminated by applying multi-omic analyses. Moreover, the CA extract loaded 3D scaffold also showed great ability to promote wound healing in diabetic chronic wounds. Due to its ease of preparation, low-cost, biosafety, and therapeutic outcomes, this work proposes an effective strategy for promoting chronic wound healing.
如何促进伤口愈合仍然是医疗保健中的一个主要挑战，而巨噬细胞是愈合过程的关键组成部分。与各种生物活性药物相比，许多植物已被报道通过调节伤口的免疫反应来促进伤口愈合过程。在这项工作中，开发了一种负载天然积雪草提取物（CA 提取物）的 3D 打印水凝胶支架用于伤口愈合。这种 CA@3D 支架使用明胶（Gel）和海藻酸钠（SA）与 CA 提取物作为 3D 打印的生物墨水。CA 提取物含有多种生物活性化合物，使积雪草中的各种有效成分协同作用。与商业伤口敷料相比，打印的 CA@3D 支架可以贴合伤口形状，协调伤口内的巨噬细胞和免疫反应，促进伤口愈合。通过应用多组学分析，还阐明了促进伤口愈合的潜在机制。此外，负载 CA 提取物的 3D 支架在糖尿病慢性伤口中也显示出很好的促进伤口愈合的能力。由于其易于制备、低成本、生物安全性和治疗效果，这项工作为促进慢性伤口愈合提出了一种有效的策略。
