Chew Leon H, Mercier Eloi, Rogalski Jason C, Pippard Sara, Knock Erin
STEMCELL Technologies, Vancouver, BC, Canada.
Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada.
Front Mol Neurosci. 2024 Jan 29;16:1243499. doi: 10.3389/fnmol.2023.1243499. eCollection 2023.
The choroid plexus (ChP) is a highly vascularized tissue lining the ventricular space of the brain. The ChP generates cerebrospinal fluid (CSF) and forms a protective barrier in the central nervous system (CNS). Recently, a three-dimensional human pluripotent stem cell (hPSC)-derived ChP organoid model has been developed. This model generates cystic structures that are filled with a fluid resembling CSF and are surrounded by an epithelial layer expressing ependymal choroid plexus-specific markers. Here we describe a method to generate these choroid plexus organoids using a commercial kit and methods to extract the CSF-like fluid for use in downstream analysis.
脉络丛（ChP）是一种高度血管化的组织，衬于脑室内腔。脉络丛产生脑脊液（CSF）并在中枢神经系统（CNS）中形成保护屏障。最近，已开发出一种三维人类多能干细胞（hPSC）衍生的脉络丛类器官模型。该模型产生充满类似脑脊液的液体的囊性结构，并被表达室管膜脉络丛特异性标志物的上皮层包围。在这里，我们描述了一种使用商业试剂盒生成这些脉络丛类器官的方法，以及提取类似脑脊液的液体用于下游分析的方法。
