Department of Epidemiology and Health Statistics, Anhui Medical University, Hefei, 230032, Anhui, People's Republic of China.
Department of Epidemiology and Health Statistics, Anhui Medical University, Hefei, 230032, Anhui, People's Republic of China.
Alcohol. 2024 Nov;120:151-159. doi: 10.1016/j.alcohol.2024.02.003. Epub 2024 Feb 20.
Alcohol consumption is not uncommon among people with HIV (PWH) and may exacerbate HIV-induced intestinal damage, and further lead to dysbiosis and increased intestinal permeability. This study aimed to determine the changes in the fecal microbiota and its association with alcohol consumption in HIV-infected patients.
A cross-sectional survey was conducted between November 2021 and May 2022, and 93 participants were recruited. To investigate the alterations of alcohol misuse on fecal microbiology in HIV-infected individuals, we performed 16s rDNA gene sequencing on fecal samples from the low-to-moderate drinking (n = 21) and non-drinking (n = 72) groups.
Comparison between groups using alpha and beta diversity showed that the diversity of stool microbiota in the low-to-moderate drinking group did not differ from that of the non-drinking group (all p > 0.05). The Linear discriminant Analysis effect size (LEfSe) algorithm was used to determine the bacterial taxa associated with alcohol consumption, and the results showed altered fecal bacterial composition in HIV-infected patients who consumed alcohol; Coprobacillus, Pseudobutyrivibrio, and Peptostreptococcaceae were enriched, and Pasteurellaceae and Xanthomonadaceae were depleted. In addition, by using the Kyoto Encyclopedia of Genes and Genomes (KEGG), functional microbiome features were also found to be altered in the low-to-moderate drinking group compared to the control group, showing a reduction in metabolic pathways (p = 0.036) and cardiovascular disease pathways (p = 0.006).
Low-to-moderate drinking will change the composition, metabolism, and cardiovascular disease pathways of the gut microbiota of HIV-infected patients.
艾滋病病毒（HIV）感染者饮酒并不少见，这可能会加重 HIV 引起的肠道损伤，并进一步导致肠道菌群失调和通透性增加。本研究旨在确定 HIV 感染患者粪便微生物群的变化及其与饮酒的关系。
本研究于 2021 年 11 月至 2022 年 5 月进行了一项横断面调查，共招募了 93 名参与者。为了研究酒精滥用对 HIV 感染者粪便微生物群的影响，我们对低中度饮酒组（n=21）和非饮酒组（n=72）的粪便样本进行了 16s rDNA 基因测序。
使用 alpha 和 beta 多样性比较两组结果显示，低中度饮酒组的粪便微生物群多样性与非饮酒组无差异（均 P>0.05）。线性判别分析效应量（LEfSe）算法用于确定与饮酒相关的细菌分类群，结果显示，饮酒的 HIV 感染患者粪便细菌组成发生改变；Copro-bacillus、Pseudobutyrivibrio 和 Peptostreptococcaceae 丰度增加，而 Pasteurellaceae 和 Xanthomonadaceae 丰度减少。此外，通过京都基因与基因组百科全书（KEGG）分析，与对照组相比，低中度饮酒组的功能微生物组特征也发生了改变，代谢途径减少（P=0.036），心血管疾病途径减少（P=0.006）。
低中度饮酒会改变 HIV 感染患者肠道微生物群的组成、代谢和心血管疾病途径。
