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[骨骼肌中的硫辛酰胺脱氢酶、柠檬酸合酶和β-羟酰基辅酶A脱氢酶。VII. 绵羊、野味和家禽肌肉在-20℃冷冻和解冻对酶活性及亚细胞分布的影响]

[Lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase in skeletal muscles. VII. The influence of freezing (-20 degrees C) and thawing of muscles from sheep, game and poultry on activity and subcellular distribution].

作者信息

Gottesmann P, Hamm R

出版信息

Z Lebensm Unters Forsch. 1985 Aug;181(2):128-31. doi: 10.1007/BF01042574.

Abstract

Freezing at -20 degrees C and subsequent thawing of muscles from sheep, hare and deer, and of breast and leg muscle from chicken and duck, did not result in significant changes of the total activity of the enzymes citrate synthase and beta-hydroxy-acyl-CoA-dehydrogenase; lipoamide dehydrogenase seemed to be somewhat more labile. Freezing and thawing of muscle tissue caused a partial release of these three mitochondrial enzymes into the sarcoplasmic fluid which indicates similar freeze-damage of the inner membrane of the mitochondria to that observed with bovine and porcine muscles.

摘要

将绵羊、野兔和鹿的肌肉以及鸡和鸭的胸肌和腿肌在-20℃下冷冻并随后解冻,并未导致柠檬酸合酶和β-羟基酰基辅酶A脱氢酶的总活性发生显著变化;硫辛酰胺脱氢酶似乎更不稳定一些。肌肉组织的冷冻和解冻导致这三种线粒体酶部分释放到肌浆中,这表明线粒体内膜的冷冻损伤与牛和猪肌肉中观察到的类似。

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