Gottesmann P, Hamm R
Z Lebensm Unters Forsch. 1985 Nov;181(5):404-7. doi: 10.1007/BF01027407.
Frozen storage at -20 degrees C for three months and thawing of muscles from sheep, hare and deer, and of the breast and leg muscles from chicken and duck did not result in significant changes in the extractable total activities of the mitochondrial enzymes citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase; however there was a decrease in the total activity of lipoamide dehydrogenase except in the chicken leg muscle, where such a decrease did not occur. From the increase in the activities of the three enzymes in the muscle press juice it was concluded that - additionally to the effect of freezing and thawing itself - frozen storage results in further damage to the inner membrane of muscle mitochondria which is signalled by the release of membrane-bound enzymes. Chicken muscle mitochondria seem to be more stable against frozen storage of the tissue than the mitochondria in the muscles of the other species studied (including bovine and porcine muscle).
将绵羊、野兔和鹿的肌肉以及鸡和鸭的胸肌与腿肌在-20℃下冷冻保存三个月并解冻后，线粒体酶柠檬酸合酶和β-羟基酰基辅酶A脱氢酶的可提取总活性没有显著变化；然而，硫辛酰胺脱氢酶的总活性有所下降，但鸡腿肌除外，其硫辛酰胺脱氢酶总活性未出现下降。从肌肉压榨汁中这三种酶活性的增加可以得出结论：除了冷冻和解冻本身的影响外，冷冻保存还会进一步损害肌肉线粒体的内膜，这通过膜结合酶的释放表现出来。与所研究的其他物种（包括牛和猪的肌肉）的肌肉线粒体相比，鸡肌肉线粒体似乎对组织的冷冻保存更稳定。
