Hamm R, Gottesmann P
Z Lebensm Unters Forsch. 1985 Aug;181(2):121-7. doi: 10.1007/BF01042573.
Freezing of bovine, calf, and porcine skeletal muscles at -20 degrees C before or after rigor mortis and thawing at room temperature did not cause significant changes in the total activities of the mitochondrial enzymes lipoamide dehydrogenase, citrate synthase, and beta-hydroxyacyl-CoA-dehydrogenase. Freezing (pre or post rigor) and thawing result in a partial release of these enzymes from their binding to the inner membrane of the mitochondria. The transfer of enzyme activity into the sarcoplasmic fluid is due to damage of the mitochondrial membranes by freezing and thawing of the muscle tissue. During longer storage of the muscle at +2 degrees C the mitochondria become more labile towards freezing which may be recognised from an increased release of the enzymes (particularly in porcine muscle). Repeated freeze/thaw cycles cause an increase in the release of the three enzymes.
牛、小牛和猪的骨骼肌在尸僵前后于-20℃冷冻，并在室温下解冻，这并未导致线粒体酶硫辛酰胺脱氢酶、柠檬酸合酶和β-羟酰基辅酶A脱氢酶的总活性发生显著变化。冷冻（尸僵前或尸僵后）和解冻会导致这些酶从与线粒体内膜的结合中部分释放出来。酶活性转移到肌浆液中是由于肌肉组织冷冻和解冻对线粒体膜造成了损伤。在肌肉于+2℃下储存较长时间后，线粒体对冷冻变得更加不稳定，这可以从酶释放量增加（特别是在猪肌肉中）看出来。反复的冻融循环会导致这三种酶的释放量增加。
