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[骨骼肌中的硫辛酰胺脱氢酶、柠檬酸合酶和β-羟酰基辅酶A脱氢酶。12. 牛胴体电刺激对活性及亚细胞分布的影响]

[Lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase in skeletal muscle. 12. The influence of electric stimulation of beef carcasses on activity and subcellular distribution].

作者信息

Gottesmann P, Hamm R

出版信息

Z Lebensm Unters Forsch. 1985 Dec;181(6):478-81. doi: 10.1007/BF01140952.

DOI:10.1007/BF01140952
PMID:3841248
Abstract

The effect of electrical stimulation (ES) of beef carcasses at 450 V on the total extractable activity and subcellular distribution of the mitochondrial enzymes lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase in skeletal muscle (activities in the supernatant of a phosphate buffer extract and in muscle press juice) was studied. There was no influence of ES on the total activity and the subcellular distribution of these enzymes in the muscle tissue stored at +2 degrees C for 7 days nor did ES influence the extent of the release of the three enzymes from the mitochondria into the sarcoplasm by freezing (-20 degrees C) and thawing. From these results it can be concluded that ES does not result in an appreciable disintegration of the inner membrane of muscle mitochondria.

摘要

研究了在450V下对牛肉胴体进行电刺激(ES)对骨骼肌中线粒体酶硫辛酰胺脱氢酶、柠檬酸合酶和β-羟酰基辅酶A脱氢酶的总可提取活性及亚细胞分布的影响(在磷酸盐缓冲液提取物的上清液和肌肉压榨汁中的活性)。电刺激对在+2℃下储存7天的肌肉组织中这些酶的总活性和亚细胞分布没有影响,电刺激也不影响通过冷冻(-20℃)和解冻使这三种酶从线粒体释放到肌浆中的程度。从这些结果可以得出结论,电刺激不会导致肌肉线粒体内膜明显解体。

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1
[Lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase in skeletal muscle. 12. The influence of electric stimulation of beef carcasses on activity and subcellular distribution].[骨骼肌中的硫辛酰胺脱氢酶、柠檬酸合酶和β-羟酰基辅酶A脱氢酶。12. 牛胴体电刺激对活性及亚细胞分布的影响]
Z Lebensm Unters Forsch. 1985 Dec;181(6):478-81. doi: 10.1007/BF01140952.
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