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[骨骼肌的硫辛酰胺脱氢酶、柠檬酸合酶和β-羟酰基辅酶A脱氢酶。V. 绵羊、野味和家禽的肌肉在+2℃储存对活性和亚细胞分布的影响]

[Lipoamide dehydrogenase, citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase of skeletal muscle. V. Effect of storage of muscles from sheep, game and poultry at +2 degrees C on activity and subcellular distribution].

作者信息

Gottesmann P, Hamm R

出版信息

Z Lebensm Unters Forsch. 1984 Oct;179(4):301-4. doi: 10.1007/BF01830432.

Abstract

Postmortem storage under refrigeration (+2 degrees C) for 7 days of the semimembranosus muscles from sheep, hare and roe deer and of the breast and leg muscles from chicken and duck did not result in a significant decrease of the total activities of the mitochondrial enzymes citrate synthase and beta-hydroxyacyl-CoA-dehydrogenase but caused a loss of the activity of lipoamide dehydrogenase (except in chicken muscle). During storage of the muscle tissue, there was no detectable release of the three enzymes from the mitochondria into the sarcoplasmic fluid except in venison at the point of spoilage. Therefore, storage of muscle under our conditions does not result in notable damage of the inner membrane of the mitochondrion.

摘要

将绵羊、野兔和狍子的半膜肌以及鸡和鸭的胸肌与腿肌在冷藏(+2℃)条件下保存7天,线粒体酶柠檬酸合酶和β-羟酰基辅酶A脱氢酶的总活性并未显著降低,但硫辛酰胺脱氢酶的活性有所丧失(鸡肌肉除外)。在肌肉组织保存期间,除了变质时的鹿肉外,未检测到这三种酶从线粒体释放到肌浆中。因此,在我们的条件下保存肌肉不会导致线粒体内膜受到明显损伤。

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