Gottesmann P, Hamm R
Z Lebensm Unters Forsch. 1987 Apr;184(4):294-9. doi: 10.1007/BF01027666.
The subcellular distribution of the mitochondrial enzymes lipoamide dehydrogenase (LIPDH), citrate synthase (CS), and beta-hydroxyacyl-CoA-dehydrogenase (HADH) in bovine and porcine liver tissue was studied by measuring the enzyme activities in a phosphate buffer extract of tissue (total activity) and in liver press-juice (cell plasma). In slaughter-fresh liver most of the activity was located in the mitochondria. During storage of liver under refrigeration (+2 degrees C) for several days a large decrease in total LIPDH activity and a lesser decrease in HADH activity, but no change in CS activity were observed. There was no or only little release of the three enzymes into the cell plasma during storage; this indicates that storage of liver at +2 degrees C was not accompanied by a marked damage of mitochondria. Freezing (-20 degrees C) and thawing of bovine and porcine liver caused some losses of the total activity of HADH and particularly of LIPDH but not changes in CS activity. There was a considerable increase in the activities of LIPDH, CS, and HADH in the press juice after freezing and thawing of liver tissue. Apparently freezing of liver results in damage to the mitochondria and, therefore, in a partial release of the three enzymes from the inner membrane of the mitochondrion into the cell plasma. By storage of liver under refrigeration the mitochondria became more sensitive to freezing and thawing. Prolonged frozen-storage of liver resulted in an increased release of LIPDH, CS, and HADH into the cell plasma.
通过测量牛和猪肝组织磷酸盐缓冲液提取物（总活性）及肝压榨汁（细胞质）中的酶活性，研究了线粒体酶硫辛酰胺脱氢酶（LIPDH）、柠檬酸合酶（CS）和β-羟酰基辅酶A脱氢酶（HADH）的亚细胞分布。在屠宰后的新鲜肝脏中，大部分活性位于线粒体中。在2℃冷藏条件下储存数天的肝脏中，观察到LIPDH总活性大幅下降，HADH活性下降幅度较小，但CS活性无变化。储存期间，这三种酶没有或仅有少量释放到细胞质中；这表明在2℃储存肝脏时，线粒体没有受到明显损伤。牛和猪肝的冷冻（-20℃）和解冻导致HADH尤其是LIPDH的总活性有所损失，但CS活性没有变化。肝组织冷冻和解冻后，压榨汁中LIPDH、CS和HADH的活性显著增加。显然，肝脏冷冻会导致线粒体受损，因此，这三种酶会从线粒体内膜部分释放到细胞质中。通过在冷藏条件下储存肝脏，线粒体对冷冻和解冻变得更加敏感。肝脏长时间冷冻储存会导致LIPDH、CS和HADH更多地释放到细胞质中。
