Hamm R, Gottesmann P
Z Lebensm Unters Forsch. 1985 Oct;181(4):299-303. doi: 10.1007/BF01043089.
The influence of storage of bovine and porcine muscle at -20 degrees C for 12 months and of thawing on the total extractable activity and subcellular distribution (activities in the supernatant of a phosphate buffer extract and in the press juice of the thawed tissue) of the mitochondrial enzymes lipoamide dehydrogenase (LIPDH), citrate synthase (CS) and beta-hydroxyacyl-CoA-dehydrogenase (HADH) was studied. The total activity of LIPDH decreased during frozen storage whereas the activities of CS and HADH did not change appreciably. From the increase of CS and HADH activities in the muscle press juice it was concluded that--additionally to the effect of freezing and thawing itself--frozen storage results in further damage to the inner membrane of muscle mitochondria, which may be recognized from the increased release of membrane-bound enzymes. In this respect porcine muscle mitochondria seem to be more sensitive than bovine muscle mitochondria.
研究了牛和猪肌肉在-20℃储存12个月以及解冻对线粒体酶硫辛酰胺脱氢酶（LIPDH）、柠檬酸合酶（CS）和β-羟酰基辅酶A脱氢酶（HADH）的总可提取活性和亚细胞分布（磷酸盐缓冲液提取物上清液以及解冻组织压榨汁中的活性）的影响。LIPDH的总活性在冷冻储存期间下降，而CS和HADH的活性没有明显变化。从肌肉压榨汁中CS和HADH活性的增加可以得出结论，除了冷冻和解冻本身的影响外，冷冻储存还会导致肌肉线粒体内膜进一步受损，这可以从膜结合酶释放增加中看出。在这方面，猪肌肉线粒体似乎比牛肌肉线粒体更敏感。
