M R Keerthanaa, Panicker Lakshmi R, Narayan Roger, Kotagiri Yugender Goud
Department of Chemistry, Indian Institute of Technology Palakkad Palakkad Kerala 678 557 India
Department of Biomedical Engineering, NC State University Raleigh NC 27695 USA.
RSC Adv. 2024 Feb 27;14(10):7131-7141. doi: 10.1039/d4ra00110a. eCollection 2024 Feb 21.
Neurological disorders can occur in the human body as a result of nano-level variations in the neurotransmitter levels. Patients affected by neuropsychiatric disorders, that are chronic require continuous monitoring of these neurotransmitter levels for effective disease management. The current work focus on developing a highly sensitive and personalized sensor for continuous monitoring of dopamine. Here we propose a wearable microneedle-based electrochemical sensor, to continuously monitor dopamine in interstitial fluid (ISF). A chitosan-protected hybrid nanomaterial FeO-GO composite has been used as a chemical recognition element protected by Nafion antifouling coating layer. The morphological and physiochemical characterizations of the nanocomposite were carried out with XRD, XPS, FESEM, EDAX and FT-IR. The principle of the developed sensor relies on orthogonal detection of dopamine with square wave voltammetry and chronoamperometric techniques. The microneedle sensor array exhibited an attractive analytical performance toward detecting dopamine in phosphate buffer and artificial ISF. The limit of detection (LOD) of the developed sensor was observed to be low, 90 nM in square wave voltammetry and 0.6 μM in chronoamperometric analysis. The practical applicability of the microneedle sensor array has been demonstrated on a skin-mimicking phantom gel model. The microneedle sensor also exhibited good long-term storage stability, reproducibility, and sensitivity. All of these promising results suggest that the proposed microneedle sensor array could be reliable for the continuous monitoring of dopamine.
由于神经递质水平的纳米级变化，人体可能会出现神经系统疾病。患有慢性神经精神疾病的患者需要持续监测这些神经递质水平，以便有效地管理疾病。当前的工作重点是开发一种用于持续监测多巴胺的高灵敏度个性化传感器。在此，我们提出一种基于可穿戴微针的电化学传感器，用于持续监测间质液（ISF）中的多巴胺。一种壳聚糖保护的杂化纳米材料FeO-GO复合材料已被用作受Nafion防污涂层保护的化学识别元件。用XRD、XPS、FESEM、EDAX和FT-IR对纳米复合材料进行了形态学和物理化学表征。所开发传感器的原理依赖于用方波伏安法和计时电流法对多巴胺进行正交检测。微针传感器阵列在检测磷酸盐缓冲液和人工间质液中的多巴胺时表现出有吸引力的分析性能。所开发传感器的检测限（LOD）较低，方波伏安法中为90 nM，计时电流分析中为0.6 μM。微针传感器阵列的实际适用性已在皮肤模拟体模凝胶模型上得到证明。微针传感器还表现出良好的长期储存稳定性、重现性和灵敏度。所有这些有前景的结果表明，所提出的微针传感器阵列对于多巴胺的持续监测可能是可靠的。
