Wang Hailong, Li Haixia, Lu Zhenxiang, Li Wenchao, Guo Weina
College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China.
Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang 233100, China.
Pathogens. 2025 Jun 25;14(7):634. doi: 10.3390/pathogens14070634.
is an opportunistic pathogen that has been isolated from humans, pigs, and chickens, but with no reports in geese until now. This research aimed to isolate and identify from four geese, and establish a specific PCR detection method for . Strain E1 was identified as through a combination of Gram staining (Gram-positive coccus), colony morphology (α-hemolysis), and whole genome sequencing analysis. Comparative genomics was used to analyze the genome sequences of five reference strains of to screen for a species-specific genomic region (401 bp). Based on this region, specific primers were designed to establish the PCR detection method for , and the specificity and sensitivity of this assay were tested. The results showed that the target sequence was specifically amplified only for the genome of , and that the minimum nucleic acid detection concentration was 7.08 × 10 ng/μL. The mouse infection model indicated that the target fragment could be amplified from the tissue samples of dead mice in the challenge groups, verifying the applicability of PCR for clinical sample detection. Specific sequences of were detected in the lungs of three pigs using the PCR method, confirmed to be consistent through whole genome sequencing, and previously identified as or by 16S rRNA sequencing. For the detection of fecal samples from geese, canines, and felines using the PCR method, the highest positive rate was 36.9% (31/84) of geese, followed by 21.7% (20/90) of felines, and finally 6.9% (16/230) of canines. A strain of was isolated and identified in geese for the first time, and a species-specific PCR detection method for was established with high specificity and sensitivity, which could well distinguish the bacterial species from its phylogenetically related species, .
是一种机会致病菌，已从人、猪和鸡中分离出来，但迄今为止在鹅中尚无报道。本研究旨在从四只鹅中分离和鉴定，并建立针对的特异性PCR检测方法。通过革兰氏染色（革兰氏阳性球菌）、菌落形态（α溶血）和全基因组测序分析相结合，将菌株E1鉴定为。利用比较基因组学分析了五个参考菌株的基因组序列，以筛选物种特异性基因组区域（401 bp）。基于该区域设计了特异性引物，建立了针对的PCR检测方法，并对该检测方法的特异性和敏感性进行了测试。结果表明，仅针对的基因组特异性扩增出目标序列，最低核酸检测浓度为7.08×10 ng/μL。小鼠感染模型表明，在攻毒组死亡小鼠的组织样本中可扩增出目标片段，验证了PCR在临床样本检测中的适用性。使用PCR方法在三头猪的肺中检测到的特异性序列，通过全基因组测序确认一致，并且先前通过16S rRNA测序鉴定为或。对于使用PCR方法检测鹅、犬和猫的粪便样本，鹅的最高阳性率为36.9%（31/84），其次是猫的21.7%（20/90），最后是犬的6.9%（16/230）。首次在鹅中分离鉴定出一株，建立了具有高特异性和敏感性的针对的物种特异性PCR检测方法，该方法能够很好地区分细菌物种与其系统发育相关物种。
