Ma Panpan, Li Jilian, Sun Guoqing, Zhu Jianbo
College of Life Sciences, Shihezi University, Shihezi, China.
Xinjiang Production & Construction Group Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization, Biotechnology Research Institute, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China.
Front Plant Sci. 2024 Feb 14;15:1283912. doi: 10.3389/fpls.2024.1283912. eCollection 2024.
, a succulent euhalophyte of the Chenopodiaceae family, intermittently spread around northern Xinjiang, China, has the ability to grow and develop in saline and alkali environments. The objective of this study was therefore to investigate the underlying molecular mechanisms of S. dendroides response to high salt conditions. 27 sequencing libraries prepared from low salt (200 mM NaCl) and high salt (800 mM NaCl) treated plants at 5 different stages were sequenced using Illumina Hiseq 2000. A total of 133,107 unigenes were obtained, of which 4,758 were DEGs. The number of DEGs in the high salt group (3,189) was more than the low salt treatment group (733) compared with the control. GO and KEGG analysis of the DEGs at different time points of the high salt treatment group showed that the genes related to cell wall biosynthesis and modification, plant hormone signal transduction, ion homeostasis, organic osmolyte accumulation, and reactive oxygen species (ROS) detoxification were significantly expressed, which indicated that these could be the main mechanisms of acclimate to high salt stress. The study provides a new perspective for understanding the molecular mechanisms of halophytes adapting to high salinity. It also provides a basis for future investigations of key salt-responsive genes in .
梭梭是藜科多汁真盐生植物，在中国新疆北部呈间断分布，具有在盐碱环境中生长发育的能力。因此，本研究的目的是探究梭梭对高盐条件响应的潜在分子机制。使用Illumina Hiseq 2000对从低盐（200 mM NaCl）和高盐（800 mM NaCl）处理的植物在5个不同阶段制备的27个测序文库进行测序。共获得133,107个单基因，其中4,758个是差异表达基因（DEG）。与对照相比，高盐组中的DEG数量（3,189个）多于低盐处理组（733个）。对高盐处理组不同时间点的DEG进行GO和KEGG分析表明，与细胞壁生物合成和修饰、植物激素信号转导、离子稳态、有机渗透物积累和活性氧（ROS）解毒相关的基因显著表达，这表明这些可能是梭梭适应高盐胁迫的主要机制。该研究为理解盐生植物适应高盐度的分子机制提供了新的视角。它也为未来对梭梭中关键盐响应基因进行研究提供了基础。
