Asha Srinivasan, Kattupalli Divya, Vijayanathan Mallika, Soniya E V
Transdisciplinary Biology, Plant Disease Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala India.
Present Address: Department of Molecular Biology and Biotechnology, College of Agriculture, Vellayani, Kerala Agricultural University, Thiruvananthapuram, India.
Physiol Mol Biol Plants. 2024 Jan;30(1):33-47. doi: 10.1007/s12298-024-01414-z. Epub 2024 Feb 11.
Nitric oxide plays a significant role in the defense signaling during pathogen interaction in plants. Quick wilt disease is a devastating disease of black pepper, and leads to sudden mortality of pepper vines in plantations. In this study, the role of nitric oxide was studied during infection in black pepper variety Panniyur-1. Nitric oxide was detected from the different histological sections of infected leaves. Furthermore, the genome-wide transcriptome analysis characterized typical domain architect and structural features of nitrate reductase () and nitric oxide associated 1 () gene that are involved in nitric oxide biosynthesis in black pepper. Despite the upregulation of nitrate reductase (), a reduced expression of was detected in the infected black pepper leaf. Subsequent sRNAome-assisted in silico analysis revealed possible microRNA mediated regulation of mRNAs. Furthermore, sRNA/miRNA mediated cleavage on mRNA was validated through modified 5' RLM RACE experiments. Several hormone-responsive cis-regulatory elements involved in stress response was detected from the promoter regions of and genes. Our results revealed the role of nitric oxide during stress response of infection in black pepper, and key genes involved in nitric oxide biosynthesis and their post-transcriptional regulatory mechanisms.
The online version contains supplementary material available at 10.1007/s12298-024-01414-z.
一氧化氮在植物病原体相互作用期间的防御信号传导中发挥重要作用。快速枯萎病是胡椒的一种毁灭性病害，会导致种植园中的胡椒藤突然死亡。在本研究中，研究了一氧化氮在胡椒品种Panniyur-1感染过程中的作用。从受感染叶片的不同组织学切片中检测到了一氧化氮。此外，全基因组转录组分析表征了参与胡椒一氧化氮生物合成的硝酸还原酶（）和一氧化氮相关1（）基因的典型结构域架构和结构特征。尽管硝酸还原酶（）上调，但在受感染的胡椒叶片中检测到表达降低。随后的小RNA组辅助计算机分析揭示了可能的microRNA介导的mRNA调控。此外，通过改良的5'RLM RACE实验验证了sRNA/miRNA对mRNA的切割作用。从和基因的启动子区域检测到了几个参与应激反应的激素反应性顺式调控元件。我们的结果揭示了一氧化氮在胡椒感染应激反应中的作用，以及参与一氧化氮生物合成的关键基因及其转录后调控机制。
在线版本包含可在10.1007/s12298-024-01414-z获取的补充材料。
