Zhang Yiran, Qiao Ziyu, Yu Jiale, Shi Chenhong, Quan Rui, Zhang Wen, Bi Ran, Li Hongliang, Qian Wentao, Wang Menghui, Li Yixuan
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100083, China.
Mengniu Hi-Tech Dairy Products (Beijing) Co., Ltd., Beijing 101100, China.
Nutrients. 2024 Feb 27;16(5):664. doi: 10.3390/nu16050664.
Colostrum basic protein (CBP) is a trace protein extracted from bovine colostrum. Previous studies have shown that CBP can promote bone cell differentiation and increase bone density. However, the mechanism by which CBP promotes bone activity remains unclear. This study investigated the mechanism of the effect of CBP on bone growth in mice following dietary supplementation of CBP at doses that included 0.015%, 0.15%, 1.5%, and 5%. Compared with mice fed a normal diet, feeding 5% CBP significantly enhanced bone rigidity and improved the microstructure of bone trabeculae. Five-percent CBP intake triggered significant positive regulation of calcium metabolism in the direction of bone calcium accumulation. The expression levels of paracellular calcium transport proteins CLDN2 and CLDN12 were upregulated nearly 1.5-fold by 5% CBP. We conclude that CBP promotes calcium absorption in mice by upregulating the expression of the calcium-transporting paracellular proteins CLND2 and CLND12, thereby increasing bone density and promoting bone growth. Overall, CBP contributes to bone growth by affecting calcium metabolism.
初乳碱性蛋白（CBP）是一种从牛初乳中提取的微量蛋白质。先前的研究表明，CBP可促进骨细胞分化并增加骨密度。然而，CBP促进骨骼活性的机制仍不清楚。本研究调查了在饮食中补充0.015%、0.15%、1.5%和5%剂量的CBP后，CBP对小鼠骨骼生长影响的机制。与喂食正常饮食的小鼠相比，喂食5% CBP可显著增强骨骼硬度并改善骨小梁的微观结构。摄入5% CBP引发了钙代谢朝着骨钙积累方向的显著正向调节。5% CBP使细胞旁钙转运蛋白CLDN2和CLDN12的表达水平上调了近1.5倍。我们得出结论，CBP通过上调细胞旁钙转运蛋白CLND2和CLND12的表达来促进小鼠的钙吸收，从而增加骨密度并促进骨骼生长。总体而言，CBP通过影响钙代谢促进骨骼生长。
