Segneanu Adina-Elena, Vlase Gabriela, Vlase Titus, Bejenaru Ludovic Everard, Mogoşanu George Dan, Buema Gabriela, Herea Dumitru-Daniel, Ciocîlteu Maria Viorica, Bejenaru Cornelia
Institute for Advanced Environmental Research, West University of Timişoara (ICAM-WUT), 4 Oituz Street, 300086 Timişoara, Timiş County, Romania.
Research Center for Thermal Analyzes in Environmental Problems, West University of Timişoara, 16 Johann Heinrich Pestalozzi Street, 300115 Timişoara, Timiş County, Romania.
Antibiotics (Basel). 2024 Sep 23;13(9):911. doi: 10.3390/antibiotics13090911.
: , a medicinal plant used in Romanian ethnopharmacology, has been proven to have remarkable biological activity. The escalating concerns surrounding antimicrobial resistance led to a special attention being paid to new efficient antimicrobial agents based on medicinal plants and nanotechnology. We report the preparation of a novel, simple phytocarrier that harnesses the bioactive properties of and silver nanoparticles (HS-Ag system). : 's low metabolic profile was determined through gas chromatography-mass spectrometry and electrospray ionization-quadrupole time-of-flight-mass spectrometry. The morphostructural properties of the innovative phytocarrier were analyzed by X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, dynamic light scattering, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The antioxidant activity was evaluated using total phenolic content, ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) in vitro assays. The antimicrobial activity screening against , , , and was conducted using the agar well diffusion method. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay estimated the in vitro potential cytotoxicity on normal human dermal fibroblasts (NHDF) and cervical cancer (HeLa) cells. : A total of 88 biomolecules were detected, such as terpenoids, flavonoids, phenolic acids, coumarins, phenylpropanoids, iridoids, amino acids, phytosterols, fatty acids. The HS-Ag phytocarrier heightened efficacy in suppressing the growth of all tested bacterial strains compared to and exhibited a significant inhibition of HeLa cell viability. : The new HS-Ag phytocarrier system holds promise for a wide range of medical applications. The data confirm the capacity to augment the pertinent theoretical understanding in the innovative field of antimicrobial agents.
在罗马尼亚民族药理学中使用的药用植物已被证明具有显著的生物活性。围绕抗菌耐药性的担忧不断升级，使得基于药用植物和纳米技术的新型高效抗菌剂受到特别关注。我们报告了一种新型、简单的植物载体的制备，该载体利用了[植物名称]和银纳米颗粒的生物活性特性（HS-Ag系统）。通过气相色谱-质谱联用和电喷雾电离-四极杆飞行时间质谱法测定了[植物名称]的低代谢谱。通过X射线衍射、傅里叶变换红外光谱、拉曼光谱、动态光散射、扫描电子显微镜和能量色散X射线光谱分析了创新植物载体的形态结构特性。使用总酚含量、铁还原抗氧化能力和2,2-二苯基-1-苦基肼（DPPH）体外试验评估抗氧化活性。使用琼脂孔扩散法对[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]和[具体细菌名称4]进行抗菌活性筛选。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验评估了对正常人皮肤成纤维细胞（NHDF）和宫颈癌（HeLa）细胞的体外潜在细胞毒性。共检测到88种生物分子，如萜类、黄酮类、酚酸类、香豆素类、苯丙素类、环烯醚萜类、氨基酸、植物甾醇、脂肪酸。与[植物名称]相比，HS-Ag植物载体在抑制所有测试细菌菌株生长方面具有更高的功效，并对HeLa细胞活力表现出显著抑制作用。新型HS-Ag植物载体系统在广泛的医学应用中具有潜力。这些数据证实了在抗菌剂创新领域增强相关理论理解的能力。
