Petrocheilou V, Richmond M H, Bennett P M
Antimicrob Agents Chemother. 1979 Aug;16(2):225-30. doi: 10.1128/AAC.16.2.225.
This paper describes an extension to an earlier account of the coliform flora carried by a married couple, one of whom was taking tetracycline for prolonged periods. The latter phase of this study was notable for the following: first, certain tetracycline-resistant Escherichia coli O antigen types persisted in one of the participants for several weeks after tetracycline was withdrawn; second, a course of ampicillin led to replacement of the tetracycline-resistant flora by one that was ampicillin resistant, but the end of the ampicillin course led to the reappearance of the tetracycline-resistant line, even though no tetracycline was being taken; and third, the tetracycline-sensitive O75 E. coli, which appeared toward the end of the survey, had not lost their plasmid but carried a derivative in which the tetracycline resistance gene(s) had been inactivated by the insertion of an extra piece of deoxyribonucleic acid with a molecular weight of about 1 megadalton.
本文描述了对一对已婚夫妇携带的大肠菌群菌群早期研究的扩展，其中一人长期服用四环素。本研究的后期阶段有以下显著特点：第一，某些耐四环素的大肠杆菌O抗原类型在停药后在其中一名参与者体内持续存在数周；第二，一个疗程的氨苄青霉素导致耐四环素菌群被耐氨苄青霉素的菌群取代，但氨苄青霉素疗程结束后，即使没有服用四环素，耐四环素的菌株也再次出现；第三，在调查接近尾声时出现的对四环素敏感的O75大肠杆菌，并未丢失其质粒，而是携带了一种衍生物，其中四环素抗性基因已因插入一段分子量约为1兆道尔顿的额外脱氧核糖核酸而失活。
