用于检测E型肉毒梭菌毒素的酶联免疫吸附测定法。
Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin.
作者信息
Notermans S, Dufrenne J, Kozaki S
出版信息
Appl Environ Microbiol. 1979 Jun;37(6):1173-5. doi: 10.1128/aem.37.6.1173-1175.1979.
Abstract
The enzyme-linked immunosorbent assay using the "double-sandwich" technique was utilized to determine Clostridium botulinum type E toxin. With this technique, about 80 mouse intraperitoneal 50% lethal doses of toxin could be detected. Cross-reaction was hardly observed with C. botulinum type A and B toxins. No cross-reaction was observed with culture supernatants of C. botulinum type C or other Clostridium strains. In all probability this was due to the high specificity of the antiserum prepared aginst the toxic component of type E toxin.
摘要
采用“双夹心”技术的酶联免疫吸附测定法用于测定E型肉毒梭菌毒素。利用该技术，可检测到约80个小鼠腹腔注射50%致死剂量的毒素。几乎未观察到与A型和B型肉毒梭菌毒素的交叉反应。未观察到与C型肉毒梭菌培养上清液或其他梭菌菌株的交叉反应。这很可能是由于针对E型毒素毒性成分制备的抗血清具有高度特异性。
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