通过酶联免疫吸附测定法检测G型肉毒梭菌毒素
Detection of Clostridium botulinum type G toxin by enzyme-linked immunosorbent assay.
作者信息
Lewis G E, Kulinski S S, Reichard D W, Metzger J F
出版信息
Appl Environ Microbiol. 1981 Dec;42(6):1018-22. doi: 10.1128/aem.42.6.1018-1022.1981.
Abstract
Clostridium botulinum type G toxin was detected and quantified readily with the enzyme-linked immunosorbent assay. With the double-sandwich technique and alkaline phosphatase as the enzyme indicator, C. botulinum toxin type G was detected in quantities equaling those required for one mouse intraperitoneal median lethal dose. The time required for the procedure was approximately 6.5 h, but this requirement could have been reduced to 5.5 h or less with the use of precoated plates stored at -70 degrees C. Cross-reactions did not occur with culture extracts of C. sporogenes of C. botulinum types B, C, D, E, and F. Acidic preparations of C. botulinum type A exhibited nonspecific reactivity. Likewise, 50% of the C. subterminale isolates tested were cross-reactive in the assay. These latter isolates express similar metabolic and physiological characteristics with C. botulinum type G.
摘要
通过酶联免疫吸附测定法能够轻松检测并定量G型肉毒梭菌毒素。采用双抗体夹心法并以碱性磷酸酶作为酶指示剂，所检测出的G型肉毒梭菌毒素量等同于一只小鼠腹腔注射半数致死剂量所需的毒素量。该检测过程所需时间约为6.5小时，但如果使用保存在-70摄氏度的预包被板，所需时间可缩短至5.5小时或更短。B、C、D、E和F型肉毒梭菌的生孢梭菌培养提取物未出现交叉反应。A型肉毒梭菌的酸性制剂表现出非特异性反应。同样，所测试的50%的近未端梭菌分离株在该检测中具有交叉反应性。这些后期分离株与G型肉毒梭菌表现出相似的代谢和生理特征。
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