Longobardi Consiglia, Damiano Sara, Vaccaro Emanuela, Ballistreri Gabriele, Restucci Brunella, Paciello Orlando, Florio Salvatore, Ciarcia Roberto
Veterinary Medicine and Animal Productions Department, University of Naples "Federico II", 80137 Napoli, Italy.
Council for Agricultural Research and Economics (CREA), Research Centre for Olive, Fruit and Citrus Crops, Corso Savoia, 190, 95024 Acireale, Italy.
Antioxidants (Basel). 2024 Feb 27;13(3):289. doi: 10.3390/antiox13030289.
Ochratoxin A (OTA) is a highly potent mycotoxin that contaminates many kinds of food and feed sources. Its significant impact on human health and animal productivity makes it a topic of particular concern. The role of specific bioactive compounds used as dietary antioxidants is believed to be substantial due to their capacity to act as free radical scavengers. Because of the well-known oxidative stress induced by OTA, the primary objective of this work was to evaluate the antioxidant effects of a standardized powder extract recovered from citrus processing waste, red orange and lemon extract (RLE), on liver damage induced by OTA in a rat model. This study aimed to examine the impact of oral administration of RLE (90 mg/kg b.w.) on hepatic function and oxidative balance in Sprague-Dawley rats ( = 6/group) treated with OTA (0.5 mg/kg b.w.) over a period of 14 days. The administration of OTA alone resulted in both biochemical changes and an imbalance in redox status in the liver. However, the use of RLE alleviated the activity of antioxidant enzymes and dramatically decreased the serum levels of ALT (alanine aminotransferase), AST (aspartate aminotransferase), and ALP (alkaline phosphatase), providing evidence of its protective benefits. Based on the findings from liver histology tests, the administration of RLE resulted in mitigation of lymphoplasmacytic inflammation, steatosis, and necrosis in the OTA group. These results indicate that the novel phytoextract RLE holds potential for application in the field of nutraceuticals.
赭曲霉毒素A（OTA）是一种极具毒性的霉菌毒素，会污染多种食品和饲料来源。它对人类健康和动物生产力有重大影响，因此成为特别受关注的话题。由于特定生物活性化合物具有作为自由基清除剂的能力，人们认为它们作为膳食抗氧化剂的作用很大。鉴于OTA会引发众所周知的氧化应激，这项工作的主要目的是评估从柑橘加工废料中提取的标准化粉末提取物——红橙和柠檬提取物（RLE）对OTA诱导的大鼠肝脏损伤的抗氧化作用。本研究旨在考察口服RLE（90毫克/千克体重）对经OTA（0.5毫克/千克体重）处理14天的斯普拉格-道利大鼠（每组 = 6只）肝脏功能和氧化平衡的影响。单独给予OTA会导致肝脏出现生化变化和氧化还原状态失衡。然而，使用RLE可提高抗氧化酶的活性，并显著降低血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）和碱性磷酸酶（ALP）的水平，证明了其保护作用。基于肝脏组织学检查结果，给予RLE可减轻OTA组的淋巴细胞浆细胞炎症、脂肪变性和坏死。这些结果表明，新型植物提取物RLE在营养保健品领域具有应用潜力。
