使用微ID系统与API 20E及传统培养基对肠杆菌科细菌进行快速鉴定。
Rapid identification of Enterobacteriaceae with the micro-ID system versus API 20E and conventional media.
作者信息
Barry A L, Badal R E
出版信息
J Clin Microbiol. 1979 Sep;10(3):293-8. doi: 10.1128/jcm.10.3.293-298.1979.
The Micro-ID system for rapid (4 h) identification of Enterobacteriaceae was evaluated by testing 433 enteric bacilli and 9 other gram-negative bacilli. Each isolate was identified with conventional tubed media and was also tested in the Micro-ID and API 20E systems. The overall accuracy of both systems was 97%. Micro-ID tests for the Voges-Proskauer reaction, indole and H2S production, and ornithine and lysine decarboxylase all demonstrated a 97 to 99% correlation with conventional methods. Only 86% of the Micro-ID urease tests agreed with Christenson urea agar. Two inoculum densities were tested in Micro-ID panels, with 157 stock cultures. Over 90% of the tests were unaffected by changes in inoculum density. Tests with four control strains suggested that the Micro-ID system was more reproducible when a light inoculum was used. The Micro-ID system was found to be a very convenient method for rapid, accurate, and precise identification of the Enterobacteriaceae.
通过检测433株肠道杆菌和9株其他革兰氏阴性杆菌，对用于快速（4小时）鉴定肠杆菌科细菌的Micro-ID系统进行了评估。每种分离菌先用传统的试管培养基进行鉴定，同时也在Micro-ID和API 20E系统中进行检测。两个系统的总体准确率均为97%。Micro-ID对Voges-Proskauer反应、吲哚和硫化氢产生以及鸟氨酸和赖氨酸脱羧酶的检测结果与传统方法的相关性均达97%至99%。Micro-ID脲酶试验只有86%与克里斯滕森尿素琼脂试验结果一致。在Micro-ID鉴定板中对两种接种密度进行了检测，共检测了157株菌株。超过90%的试验不受接种密度变化的影响。对四株对照菌株的检测表明，使用轻接种量时Micro-ID系统的重复性更好。Micro-ID系统是一种非常方便的方法，可用于快速、准确和精确地鉴定肠杆菌科细菌。
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