Barry A L, Gavan T L, Smith P B, Matsen J M, Morello J A, Sielaff B H
J Clin Microbiol. 1982 Jun;15(6):1111-9. doi: 10.1128/jcm.15.6.1111-1119.1982.
Gram-negative bacilli were identified within 3 to 6 h by determining susceptibility to 18 different antibacterial agents in the Autobac I system and by applying a two-stage quadratic discriminant analysis to the susceptibility patterns. The Autobac system was compared with standard reference methods for identifying glucose nonfermenters and glucose fermenters. Intralaboratory and interlaboratory precision of the Autobac system was comparable to that of the reference methods. Sensitivity (accuracy) and specificity of the two systems were also comparable, although there were some differences with certain species. Autobac responses were considered to be equivocal (needing additional tests) if the relative probability of an accurate identification was less than 0.70. Only 5% of 2,889 strains produced such equivocal results; a similar number of strains gave low probability levels with the reference methods. When the two systems disagreed, an independent reference laboratory arbitrated, confirming 49% of the Autobac responses and 36% of the reference identifications. With equivocal responses excluded, the overall accuracy of the Autobac system was 95.3% compared with 95.9% for the reference method. The respective accuracy estimates would be 93.8% and 93.1% if all first-choice identifications were evaluated.
通过在自动细菌鉴定仪I系统中测定对18种不同抗菌剂的敏感性，并对药敏模式应用两阶段二次判别分析，在3至6小时内鉴定出革兰氏阴性杆菌。将自动细菌鉴定系统与鉴定葡萄糖非发酵菌和葡萄糖发酵菌的标准参考方法进行比较。自动细菌鉴定系统在实验室内和实验室间的精密度与参考方法相当。两个系统的敏感性（准确性）和特异性也相当，尽管在某些菌种上存在一些差异。如果准确鉴定的相对概率小于0.70，则自动细菌鉴定仪的结果被认为是不明确的（需要额外测试）。在2889株菌株中，只有5%产生了这种不明确的结果；参考方法也有类似数量的菌株给出低概率水平。当两个系统结果不一致时，由一个独立的参考实验室进行仲裁，自动细菌鉴定仪的结果有49%得到确认，参考鉴定结果有36%得到确认。排除不明确的结果后，自动细菌鉴定系统的总体准确率为95.3%，而参考方法为95.9%。如果评估所有首选鉴定结果，各自的准确率估计分别为93.8%和93.1%。
