Ryntathiang Iadalin, Dharmalingam Jothinathan Mukesh Kumar, Behera Archana, Saravanan Saantosh, Murugan Ramadurai
Centre for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, IND.
Cureus. 2024 Mar 11;16(3):e55933. doi: 10.7759/cureus.55933. eCollection 2024 Mar.
Aim This study involves synthesizing metal nanoparticles (NPs) via the green synthesis method using leaf, bark, and peel and comparatively evaluating their antibacterial activity in vitro through the analysis of cobalt oxide NPs (CoNPs), copper NPs (CuNPs), and selenium NPs (SeNPs). This research contributes to eco-friendly approaches for producing functional nanomaterials with potential applications in medicine and environmental remediation. Materials and methods The metal NPs were synthesized using leaf, bark, and peel. These leaf extracts act as self-reducing and stabilizing agents. The antibacterial activity was assessed by the well diffusion method. Cultures of pathogenic bacteria species such as , , , and were prepared. NPs were applied to the culture, and zones of inhibition (ZOIs) were measured. The data were statistically analyzed to compare the antibacterial efficacy of the different NPs. Results The successfully synthesized CoNPs, CuNPs, and SeNPs showed distinctive phytochemical properties. CoNPs exhibited the highest ZOI against most bacterial strains, with CuNPs and SeNPs following. CoNPs consistently showed superior performance compared to CuNPs and SeNPs. Conclusion Our study analyzed the bioactivity of metal NPs produced using green synthesis with plant extracts. CoNPs have shown superior antibacterial effectiveness against both Gram-positive and Gram-negative bacteria when compared to CuNPs and SeNPs. This may be due to their larger surface area, smaller size, unique electrical, magnetic, and catalytic properties, as well as their improved contact with the bacterial cell wall and membrane.
目的 本研究涉及通过使用树叶、树皮和果皮的绿色合成方法来合成金属纳米颗粒（NPs），并通过分析氧化钴纳米颗粒（CoNPs）、铜纳米颗粒（CuNPs）和硒纳米颗粒（SeNPs）在体外比较评估它们的抗菌活性。本研究有助于采用生态友好型方法生产在医学和环境修复方面具有潜在应用的功能性纳米材料。材料与方法 使用树叶、树皮和果皮合成金属纳米颗粒。这些树叶提取物充当自还原剂和稳定剂。通过平板扩散法评估抗菌活性。制备了诸如 、 、 和 等病原菌的培养物。将纳米颗粒应用于培养物，并测量抑菌圈（ZOIs）。对数据进行统计分析以比较不同纳米颗粒的抗菌效果。结果 成功合成的CoNPs、CuNPs和SeNPs表现出独特的植物化学性质。CoNPs对大多数细菌菌株表现出最高的抑菌圈，其次是CuNPs和SeNPs。与CuNPs和SeNPs相比，CoNPs始终表现出更优异的性能。结论 我们的研究分析了使用植物提取物通过绿色合成制备的金属纳米颗粒的生物活性。与CuNPs和SeNPs相比，CoNPs对革兰氏阳性菌和革兰氏阴性菌均表现出更优异的抗菌效果。这可能是由于它们更大的表面积、更小的尺寸、独特的电学、磁学和催化性质，以及它们与细菌细胞壁和细胞膜的更好接触。
