Preparing and evaluating the anti-microbial effect of mouthwash on some of the most common oral microorganisms.

BACKGROUND

Due to the increasing resistance of bacteria to antibiotics and anti-bacterial compounds in plants, Boiss plant extract can be used in mouthwash compounds with its anti-microbial activity.

METHODS AND MATERIALS

The anti-bacterial and anti-fungal activity of mouthwash was investigated on , , and , and . To analyse the anti-microbial effect of this mouthwash, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the broth microdilution method.

RESULTS

The average MIC and MBC of mouthwash for were 1.56 and 3.12 (mg/ml), respectively, for , 0.25 and 0.65 (mg/ml), and for , respectively, 0.25 and 0.65 (mg/ml). The highest MIC and MBC values were for , and the MIC and MBC values were equal for and . Average MIC and MBC were determined as 2.41 and 4.16 (mg/ml) for and 2.34 and 5.72 (mg/ml) for , respectively. MIC values of mouthwash were higher for and MBC values for .

CONCLUSION

Our results showed a promising anti-fungal-anti-bacterial effect of extract. extract may be used as an alternative to chemical mouthwashes.