Biology Centre Czech Academy of Sciences, Institute of Soil Biology and BiogeochemistryNaSádkách 7, 37005 České Budějovice, Czech Republic.
University of Chemistry, and Technology, Prague, Faculty of Food and Biochemical Technology, Department of Biochemistry and Microbiology, Technická 5, 16628 Prague, Czech Republic.
Int J Syst Evol Microbiol. 2024 Apr;74(4). doi: 10.1099/ijsem.0.006335.
The taxonomic position of three actinobacterial strains, BCCO 10_0061, BCCO 10_0798, and BCCO 10_0856, recovered from bare soil in the Sokolov Coal Basin, Czech Republic, was established using a polyphasic approach. The multilocus sequence analysis based on 100 single-copy genes positioned BCCO 10_0061 in the same cluster as , strain BCCO 10_0798 in the same cluster as , , , and , and strain BCCO 10_0856 clustered together with and . Morphological and chemotaxonomic characteristics of these strains support their assignment to the genus . In all three strains, MK-9(H) accounted for more than 80 % of the isoprenoid quinone. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The whole-cell sugars were rhamnose, ribose, mannose, glucose, and galactose. The major fatty acids (>10 %) were iso-C, anteiso-C, iso-C, and C. The polar lipids were diphosphatidylglycerol, methyl-phosphatidylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol. The genomic DNA G+C content of strains (mol%) was 68.8 for BCCO 10_0061, 69.2 for BCCO 10_0798, and 68.5 for BCCO 10_0856. The combination of digital DNA-DNA hybridization results, average nucleotide identity values and phenotypic characteristics of BCCO 10_0061, BCCO 10_0798, and BCCO 10_0856 distinguishes them from their closely related strains. Bioinformatic analysis of the genome sequences of the strains revealed several biosynthetic gene clusters (BGCs) with identities >50 % to already known clusters, including BGCs for geosmin, coelichelin, ε-poly-l-lysine, and erythromycin-like BGCs. Most of the identified BGCs showed low similarity to known BGCs (<50 %) suggesting their genetic potential for the biosynthesis of novel secondary metabolites. Based on the above results, each strain represents a novel species of the genus , for which we propose the name sp. nov. for BCCO 10_0061 (=DSM 116175), sp. nov. for BCCO 10_0798 (=DSM 116176), and sp. nov. for BCCO 10_0856 (=DSM 116177).
从捷克共和国索科洛夫煤盆地裸土中分离到的三株放线菌 BCCO 10_0061、BCCO 10_0798 和 BCCO 10_0856 的分类地位采用多相方法确定。基于 100 个单拷贝基因的多位点序列分析将 BCCO 10_0061 定位在与 菌株相同的聚类中，BCCO 10_0798 与 、 、 、 和 菌株相同的聚类中，BCCO 10_0856 与 和 菌株聚类在一起。这些菌株的形态学和化学分类特征支持它们被分配到属 。在所有三株菌中，MK-9(H)占类异戊二烯醌的 80%以上。细胞壁肽聚糖中的诊断二氨基氨基酸为 -二氨基庚二酸。全细胞糖为鼠李糖、核糖、甘露糖、葡萄糖和半乳糖。主要脂肪酸（>10%）为 iso-C、anteiso-C、iso-C 和 C。极性脂为双磷脂酰甘油、甲基磷酸乙醇胺、磷脂乙醇胺、羟基磷酸乙醇胺、磷脂酰甘油和磷脂酰肌醇。菌株（mol%）的基因组 DNA G+C 含量为 BCCO 10_0061 为 68.8，BCCO 10_0798 为 69.2，BCCO 10_0856 为 68.5。BCCO 10_0061、BCCO 10_0798 和 BCCO 10_0856 的数字 DNA-DNA 杂交结果、平均核苷酸同一性值和表型特征的组合将它们与亲缘关系较近的菌株区分开来。菌株基因组序列的生物信息学分析揭示了几个生物合成基因簇（BGCs），其身份与已知簇的相似度>50%，包括 geosmin、coelichelin、ε-聚-l-赖氨酸和红霉素样 BGCs。大多数鉴定出的 BGCs 与已知 BGCs 的相似度较低（<50%），表明它们具有合成新型次生代谢物的遗传潜力。基于上述结果，每株菌均代表属 的一个新种，我们提议将 BCCO 10_0061（=DSM 116175）命名为 sp. nov.，BCCO 10_0798（=DSM 116176）命名为 sp. nov.，BCCO 10_0856（=DSM 116177）命名为 sp. nov.。
