Department of Human Nutrition, The Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Słoneczna 45F, 10-718 Olsztyn, Poland.
College of Medical Sciences in Olsztyn, Nicolaus Copernicus Superior School, Nowogrodzka 47A, 00-695 Warsaw, Poland.
Molecules. 2024 May 2;29(9):2101. doi: 10.3390/molecules29092101.
The study aimed to determine the phenolic content and antioxidant capacity of five protein supplements of plant origin. The content and profile of phenolics were determined using the UHPLC-DAD-MS method, while antioxidant capacity (ABTS and DPPH assays) and total phenolic content (TPC) were evaluated using spectrophotometric tests. In the analyzed proteins, twenty-five polyphenols were detected, including eleven phenolic acids, thirteen flavonoids, and one ellagitannin. Hemp protein revealed the highest individual phenolics content and TPC value (1620 μg/g and 1.79 mg GAE/g, respectively). Also, hemp protein showed the highest antioxidant activity determined via ABTS (9.37 μmol TE/g) and DPPH (9.01 μmol TE/g) assays. The contents of -coumaric acid, -coumaric acid, kaempferol, rutin, isorhamnetin-3--rutinoside, kaempferol-3--rutinoside, and TPC value were significantly correlated with antioxidant activity assays. Our findings indicate that plant-based protein supplements are a valuable source of phenols and can also be used in research related to precision medicine, nutrigenetics, and nutrigenomics. This will benefit future health promotion and personalized nutrition in the prevention of chronic diseases.
本研究旨在测定五种植物源蛋白质补充剂的酚类含量和抗氧化能力。采用 UHPLC-DAD-MS 法测定酚类物质的含量和分布，采用分光光度法测定抗氧化能力（ABTS 和 DPPH 法）和总酚含量（TPC）。在所分析的蛋白质中，检测到二十五种多酚类物质，包括十一种酚酸、十三种类黄酮和一种鞣花单宁。麻蛋白的单体酚类含量和 TPC 值最高（分别为 1620μg/g 和 1.79mgGAE/g）。此外，麻蛋白在 ABTS（9.37μmolTE/g）和 DPPH（9.01μmolTE/g）测定中表现出最高的抗氧化活性。-香豆酸、-香豆酸、山奈酚、芦丁、异鼠李素-3--芸香糖苷、山奈酚-3--芸香糖苷和 TPC 值与抗氧化活性测定呈显著相关。我们的研究结果表明，植物性蛋白质补充剂是酚类物质的有价值来源，也可用于与精准医学、营养遗传学和营养基因组学相关的研究。这将有助于未来促进健康和个性化营养，预防慢性病。
