传染性单核细胞增多症中的T淋巴细胞。II. 体外对白介素-2的反应及T细胞系的建立。
T lymphocytes in infectious mononucleosis. II. Response in vitro to interleukin-2 and establishment of T cell lines.
作者信息
Bishop C J, Moss D J, Ryan J M, Burrows S R
出版信息
Clin Exp Immunol. 1985 Apr;60(1):70-7.
Abstract
The addition of 20% interleukin-2 (IL-2) significantly reduced the percentage of T lymphocytes dying in vitro after being isolated from the peripheral blood of acute infectious mononucleosis (IM) patients. Moreover, the immediate addition of 20% IL-2 to freshly isolated blood allowed IM T cell lines to be readily established from the peripheral blood of acute IM patients. Characterization of seven of these IM T cell lines showed them to be T3+, T11+, T4-, T9- and generally T10-. Over half of the lines characterized were T8+. It will now be possible to re-evaluate IM T cell effector functions as previous assays of IM T cell functions may have been influenced by the presence of rapid and extensive T cell death in vitro.
摘要
添加20%的白细胞介素-2（IL-2）可显著降低从急性传染性单核细胞增多症（IM）患者外周血中分离出的T淋巴细胞在体外死亡的百分比。此外，向新鲜分离的血液中立即添加20%的IL-2，使得能够从急性IM患者的外周血中轻松建立IM T细胞系。对其中7个IM T细胞系的表征显示，它们为T3+、T11+、T4-、T9-，且通常为T10-。超过半数被表征的细胞系为T8+。由于之前对IM T细胞功能的检测可能受到体外T细胞快速广泛死亡的影响，现在将有可能重新评估IM T细胞的效应功能。
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