Novel Metabolites from the Marine-Derived Fungus sp. SCSIO41203 Show Promising In Vitro Antitumor Activity as Methuosis Inducers in PC-3 Cells.

Two new cytochalasin derivatives, peniotrinins A () and B (), three new citrinin derivatives, peniotrinins C-E (, , ), and one new tetramic acid derivative, peniotrinin F (), along with nine structurally related known compounds, were isolated from the solid culture of sp. SCSIO41203. Their structures, including the absolute configurations of their stereogenic carbons, were fully elucidated based on spectroscopic analysis, quantum chemical calculations, and the calculated ECD. Interestingly, is the first example of a rare 6/5/5/5/6/13 hexacyclic cytochalasin. We screened the above compounds for their anti-prostate cancer activity and found that compound had a significant anti-prostate cancer cell proliferation effect, while compounds and showed weak activity at 10 μM. We then confirmed that compound exerts its anti-prostate cancer effect by inducing methuosis through transmission electron microscopy and cellular immunostaining, which suggested that compound might be first reported as a potential anti-prostate methuosis inducer.