Langford M P, Stanton G J, Barber J C, Baron S
J Infect Dis. 1979 Jun;139(6):653-8. doi: 10.1093/infdis/139.6.653.
The clinical, virological, and immunological courses of a laboratory-acquired hemorrhagic conjunctivitis were followed. Coxsackievirus type A24 (CA24) was isolated from tears for only two days but from sputum and fecal samples for 20 days. Maximal levels of virus were detected in the right eye (10(5.1) 50% tissue culture infective doses/ml) 5-7 hr before conjunctivitis in the right eye, and in feces (10(2.8) 50% tissue culture infective doses/g) and sputum (10(2.5) 50% tissue culture infective doses/ml) five and eight days after onset of conjunctivitis, respectively. Specific neutralizing activity against CA24 was detected in tear samples 48-56 hr after exposure, and elimination of virus was associated with the rise in the neutralizing activity in tears. This activity was detected earlier than previously reported antibody responses of the eye. The absence of interferon and the association of this early neutralizing activity in tears with early elimination of virus from the eye suggest that neutralizing activity may play a major role in the clinical and pathological manifestations of this infection.
对一例实验室获得性出血性结膜炎的临床、病毒学和免疫学过程进行了跟踪观察。从泪液中仅在两天内分离出A24型柯萨奇病毒（CA24），但从痰液和粪便样本中分离出该病毒长达20天。在右眼结膜炎出现前5 - 7小时，右眼检测到最高病毒水平（10(5.1) 50%组织培养感染剂量/毫升），在结膜炎发病后第5天和第8天，粪便（10(2.8) 50%组织培养感染剂量/克）和痰液（10(2.5) 50%组织培养感染剂量/毫升）中分别检测到最高病毒水平。暴露后48 - 56小时在泪液样本中检测到针对CA24的特异性中和活性，病毒的清除与泪液中中和活性的升高相关。这种活性比先前报道的眼部抗体反应检测得更早。泪液中缺乏干扰素以及这种早期中和活性与病毒从眼部早期清除的关联表明，中和活性可能在这种感染的临床和病理表现中起主要作用。
