Smrkovski L L, Buck R L, Alcantara A K, Rodriguez C S, Uylangco C V
Trans R Soc Trop Med Hyg. 1985;79(1):37-41. doi: 10.1016/0035-9203(85)90228-7.
One hundred cases of slide-confirmed Plasmodium falciparum malaria admitted to the San Lazaro Hospital, Manila, Philippines were screened for in vitro resistance to chloroquine, quinine, amodiaquine and mefloquine using the microtechnique. 59 of the 100 primary parasite isolates produced schizonts, whereas the remaining 41 isolates did not. 51 of the 59 isolates tested were resistant in vitro to chloroquine and eight were sensitive. In contrast, three of the primary isolates were resistant to quinine, three showed resistance to amodiaquine and four were mefloquine-resistant. 43 of the strains judged chloroquine-resistant in vitro were fully in vitro sensitive to amodiaquine, quinine and mefloquine. One chloroquine-resistant isolate was also resistant to quinine alone. Three isolates that were resistant to chloroquine were also resistant to amodiaquine. An additional three were cross-resistant to chloroquine and mefloquine. A single isolate was found to be resistant to chloroquine, quinine and mefloquine and another was cross-resistant to chloroquine, quinine and amodiaquine. All strains demonstrating in vitro resistance to amodiaquine, quinine or mefloquine also showed in vitro resistance to chloroquine. The parasites in 22 patients showed in vivo resistance to chloroquine therapy. 86% were of the R1 type, 9% were R2 and 5% R3. All 22 patients demonstrating in vivo resistance to chloroquine showed in vitro resistance.
对菲律宾马尼拉圣拉萨罗医院收治的100例经玻片确诊的恶性疟原虫疟疾患者，采用微量技术筛选其对氯喹、奎宁、阿莫地喹和甲氟喹的体外耐药性。100株原始疟原虫分离株中，59株产生了裂殖体，其余41株未产生。在检测的59株分离株中，51株对氯喹体外耐药，8株敏感。相比之下，3株原始分离株对奎宁耐药，3株对阿莫地喹耐药，4株对甲氟喹耐药。在体外被判定为氯喹耐药的43株菌株对阿莫地喹、奎宁和甲氟喹完全敏感。1株氯喹耐药分离株也仅对奎宁耐药。3株对氯喹耐药的分离株也对阿莫地喹耐药。另外3株对氯喹和甲氟喹交叉耐药。发现1株对氯喹、奎宁和甲氟喹耐药，另1株对氯喹、奎宁和阿莫地喹交叉耐药。所有对阿莫地喹、奎宁或甲氟喹表现出体外耐药性的菌株也对氯喹表现出体外耐药性。22例患者的疟原虫对氯喹治疗表现出体内耐药性。86%为R1型，9%为R2型，5%为R3型。所有22例对氯喹表现出体内耐药性的患者均表现出体外耐药性。
