Department of Environmental and Sanitary Engineering, Faculty of Civil Engineering, Universidad del Cauca, Popayán, Colombia.
Department of Physiological Sciences, Faculty of Health Sciences, Universidad del Cauca, Popayán, Colombia.
Environ Monit Assess. 2024 Jun 15;196(7):619. doi: 10.1007/s10661-024-12764-2.
Helicobacter pylori is a microorganism that infects 60% of the population and is considered the main cause of atrophic gastritis, gastric and duodenal ulcers, and gastric cancer. Different emerging pathogens have been found in drinking water and their presence is considered to be an important public health problem. For this reason, it is necessary to carry out the validation of reliable technologies for this type of pathogens and evaluate their performance. This paper reports, for the first time, H. pylori reduction in a drinking water pilot plant of two slow sand filters (SSF). Inlet water was taken from a gravel filtration system of a rural water supply in Colombia and then inoculated with viable cells of H. pylori. By determining the Genomic Units (GU) through quantitative Polymerase Chain Reaction (qPCR), the concentration of GU/sample was measured. In the inlet water amplification for SSF1 and SSF2 were 5.13 × 10 ± 4.48 × 10 and 6.59 × 10 ± 7.32 × 10, respectively, while for the treated water they were 7.0 ± 5.6 and 2.05 × 10 ± 2.9 × 10 GU/sample for SSF1 and SSF2, respectively. The SSF pilot plant reached up to 3 log reduction units of H. pylori; therefore, since there is not an H. pylori contamination indicator and its periodic monitoring is financially complicated, the SSF could guarantee the drinking water quality necessity that exists in rural areas and small municipalities in developing countries, where infection rates and prevalence of this pathogen are high.
幽门螺杆菌是一种感染 60%人口的微生物，被认为是萎缩性胃炎、胃和十二指肠溃疡以及胃癌的主要原因。在饮用水中发现了不同的新兴病原体，它们的存在被认为是一个重要的公共卫生问题。因此，有必要对这类病原体的可靠技术进行验证，并评估其性能。本文首次报道了在两个慢砂滤池（SSF）的饮用水中去除幽门螺杆菌的情况。进水取自哥伦比亚农村供水系统的砾石过滤系统，然后接种了幽门螺杆菌的活细胞。通过定量聚合酶链反应（qPCR）确定基因组单位（GU），测量样品中 GU 的浓度。在 SSF1 和 SSF2 的进水扩增中，GU/sample 的浓度分别为 5.13×10±4.48×10 和 6.59×10±7.32×10，而在处理水中，SSF1 和 SSF2 的浓度分别为 7.0±5.6 和 2.05×10±2.9×10 GU/sample。SSF 中试工厂达到了 3 个对数减少单位的幽门螺杆菌；因此，由于没有幽门螺杆菌污染指标，而且其定期监测在财务上很复杂，因此 SSF 可以保证发展中国家农村和小市镇的饮用水质量需求，这些地区的感染率和这种病原体的流行率都很高。
