Yang Chen, Li Xiaopeng, Hu Miaomiao, Li Ting, Jiang Li, Zhang Yong
Department of Cardiovascular Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei, 230000, Anhui Province, China.
Key Laboratory of Digital Technology in Medical Diagnostics of Zhejiang Province, Dian Diagnostics Group Co., Ltd., Hangzhou, 310030, Zhejiang Province, China.
J Cardiovasc Transl Res. 2024 Dec;17(6):1240-1257. doi: 10.1007/s12265-024-10529-3. Epub 2024 Jun 24.
To examine the relationship between gut microbiota and disease development in chronic heart failure patients with different nutritional risk. The study analyzed stool samples from 62 CHF patients and 21 healthy peoples using 16S rRNA gene sequencing. CHF patients were separated into risk (n = 30) and non-risk group (n = 32) based on NRS2002 scores. Analysis methods used were LEfSe, random forest regression model, ROC curves, BugBase, PICRUSt2, metagenomeSeq. Risk group includes 11 cases of HFrEF, 6 cases of HFpEF, and 13 cases of HFmrEF. LefSe analysis confirmed that the risk group had higher levels of Enterobacter and Escherichia-Shigella. Correlation analysis revealed a negative correlation between prealbumin and Escherichia-Shigella. The presence of Enterobacter and Escherichia-Shigella worsens intestinal inflammation in CHF patients, impacting lysine metabolism by influencing its degradation metabolic function. This interference further disrupts albumin and prealbumin synthesis, leading to malnutrition in CHF patients and ultimately worsening the disease.
研究不同营养风险的慢性心力衰竭患者肠道微生物群与疾病发展之间的关系。该研究使用16S rRNA基因测序分析了62例慢性心力衰竭患者和21名健康人的粪便样本。根据NRS2002评分，将慢性心力衰竭患者分为风险组（n = 30）和非风险组（n = 32）。使用的分析方法包括LEfSe、随机森林回归模型、ROC曲线、BugBase、PICRUSt2、宏基因组序列分析。风险组包括11例射血分数降低的心力衰竭（HFrEF）患者、6例射血分数保留的心力衰竭（HFpEF）患者和13例射血分数中间值的心力衰竭（HFmrEF）患者。LEfSe分析证实，风险组中肠杆菌属和大肠埃希菌-志贺菌属水平较高。相关性分析显示，前白蛋白与大肠埃希菌-志贺菌属之间呈负相关。肠杆菌属和大肠埃希菌-志贺菌属的存在会加重慢性心力衰竭患者的肠道炎症，通过影响其降解代谢功能影响赖氨酸代谢。这种干扰进一步破坏白蛋白和前白蛋白的合成，导致慢性心力衰竭患者营养不良，最终使病情恶化。
