Żybura Adrianna, Jedziniak Piotr
Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Puławy, Poland.
J Vet Res. 2024 May 22;68(2):233-240. doi: 10.2478/jvetres-2024-0023. eCollection 2024 Jun.
The productivity of domestic animals and the safety of food products derived from them are jeopardised by mycotoxins in animal feed. To control them, feed additives are used, which limit the absorption of mycotoxins in the gastrointestinal tract of animals by binding to them. The study aimed to evaluate the effectiveness of a new model in experiments on the binding of mycotoxins from buffers and contaminated feed and to confirm the effect of a single sorbent or mixture in binding them.
Nine mineral sorbents were tested for their efficiency binding eight mycotoxins. Two experiments were conducted to indicate the mycotoxin-binding capacity of sorbents, each specifying a buffer with one of two different pH levels reflecting gastrointestinal conditions (pH 3.5 and 7.0). The first investigated the sorbent with only the buffer and mycotoxin standards, while the second did so with the sorbent, buffer and feed naturally contaminated with mycotoxins (deoxynivalenol, zearalenone, and ochratoxin A).
The sorption was significantly lower in the trial with feed. In the first experiment at gastric pH (pH 3.5), activated charcoal bound deoxynivalenol and sepiolite bound zearalenone at 70% and 96%, respectively, whereas in the second experiment with feed, the binding was only 3% and 6%.
The study underlines the challenge of finding a feed additive that would work comprehensively, binding all mycotoxins regulated by law.
家畜的生产力以及源自家畜的食品产品安全性受到动物饲料中霉菌毒素的威胁。为了控制这些霉菌毒素,人们使用饲料添加剂,这些添加剂通过与霉菌毒素结合来限制其在动物胃肠道中的吸收。本研究旨在评估一种新模型在从缓冲液和受污染饲料中结合霉菌毒素的实验中的有效性,并确认单一吸附剂或混合物在结合霉菌毒素方面的效果。
测试了九种矿物吸附剂对八种霉菌毒素的结合效率。进行了两项实验以表明吸附剂的霉菌毒素结合能力,每项实验指定一种反映胃肠道状况的两种不同pH水平之一的缓冲液(pH 3.5和7.0)。第一个实验研究仅含有缓冲液和霉菌毒素标准品的吸附剂,而第二个实验研究含有吸附剂、缓冲液和天然受霉菌毒素污染的饲料(脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮和赭曲霉毒素A)的吸附剂。
在含有饲料的试验中吸附率显著较低。在第一个实验中,在胃pH值(pH 3.5)下,活性炭对脱氧雪腐镰刀菌烯醇的结合率为70%,海泡石对玉米赤霉烯酮的结合率为96%,而在第二个含有饲料的实验中,结合率仅为3%和6%。
该研究强调了找到一种能全面发挥作用、结合所有法定霉菌毒素的饲料添加剂所面临的挑战。