有证据表明，单一的DNA连接酶参与酵母的复制和重组过程。
Evidence that a single DNA ligase is involved in replication and recombination in yeast.
作者信息
Fabre F, Roman H
出版信息
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4586-8. doi: 10.1073/pnas.76.9.4586.
Abstract
The possible existence in yeast of different nuclear DNA ligase enzymes led us to ask whether induced recombination (gene conversion) involves the same ligase as that involved in DNA replication. The conditional cdc9 mutant is known to be defective, under restrictive conditions, in the rejoining of Okazaki fragments. We show here that under the same conditions, x-ray-induced convertants within the cdc9 locus are produced with kinetics indicating that most, if not all, of the conversion events require the participation of the cdc9-controlled ligase. Thus, the same DNA ligase is involved in DNA replication and in induced gene conversion.
摘要
酵母中可能存在不同的核DNA连接酶，这促使我们去探究诱导重组（基因转换）所涉及的连接酶是否与DNA复制中涉及的连接酶相同。已知条件性cdc9突变体在限制条件下，冈崎片段的重新连接存在缺陷。我们在此表明，在相同条件下，cdc9基因座内X射线诱导的转化体产生的动力学表明，即使不是所有，大多数转换事件都需要cdc9控制的连接酶参与。因此，DNA复制和诱导基因转换涉及相同的DNA连接酶。
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