减肥手术后一个月血液中微小RNA的早期变化。
Early changes of microRNAs in blood one month after bariatric surgery.
作者信息
Lu Guanhua, Gao Huanhuan, Hu Ruixiang, Miao Ji, Dong Zhiyong, Wang Cunchuan, Chen Xinxin
机构信息
Department of Breast Surgery, The Second Affiliated Hospital of Guangzhou Medical University, No. 250, Changgang East Road, Haizhu District, Guangzhou, Guangdong Province, China.
Department of Metabolic and Bariatric Surgery, The First Affiliated Hospital of Jinan University, No. 613, Huangpu Avenue West, Tianhe District, Guangzhou, Guangdong Province, China.
出版信息
Diabetol Metab Syndr. 2024 Jul 15;16(1):163. doi: 10.1186/s13098-024-01364-2.
BACKGROUND
Changes in microRNAs (miRNAs) are relevant to bariatric surgery and its comorbidities. The characteristics of changes in miRNAs of the early postoperative period following both bariatric procedures, sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB), as well as the factors that related to the effectiveness of early weight loss remain unclear.
METHODS
We recruited 18 patients who performed SG and 15 patients who performed RYGB. Their preoperative and 1-month postoperative clinical data and fasting serum samples were collected, and the latter were analyzed by RNA-sequencing. Differential expression analysis of miRNAs was performed by the R-tool. Functional classification annotation and pathway enrichment analysis of targeted genes were analyzed by KOBAS software. The change profiles of miRNAs for both surgeries and their correlation with clinical characteristics and weight loss effectiveness were further analyzed.
RESULTS
A total of 85 differentially expressed miRNAs were identified before and after SG, while a total of 76 were found before and after RYGB. The target genes of these miRNAs were similar in the Gene Ontology enrichment analysis in SG and RYGB, and the enrichment analysis in the Kyoto Encyclopedia of Genes and Genomes was mainly related to metabolic pathways. Hsa-miR-493-5p, hsa-miR-184, and hsa-miR-3199 exhibited similar changes in SG and RYGB, and the former two were correlated with clinical characteristics. Hsa-miR-6729-5p, hsa-miR-4659b-5p, and hsa-miR-2277-5p were correlated with the weight loss effectiveness of SG, while hsa-miR-4662a-5p was correlated with the weight loss effectiveness of RYGB.
CONCLUSIONS
Short-term metabolic improvement and weight loss occurring after SG and RYGB surgery might be related to changes in miRNAs, which act on multiple biological pathways by regulating genes. In addition, some clinical characteristics and miRNAs were related to the effectiveness of early weight loss after SG and RYGB surgery.
CLINICAL TRIAL REGISTRATION
ChiCTR2200058333.
背景
微小RNA（miRNA）的变化与减肥手术及其合并症相关。减肥手术包括袖状胃切除术（SG）和Roux-en-Y胃旁路术（RYGB），术后早期miRNA的变化特征以及与早期体重减轻效果相关的因素尚不清楚。
方法
我们招募了18例行SG的患者和15例行RYGB的患者。收集他们术前和术后1个月的临床数据及空腹血清样本，并对后者进行RNA测序分析。通过R工具进行miRNA的差异表达分析。通过KOBAS软件对靶基因进行功能分类注释和通路富集分析。进一步分析两种手术的miRNA变化谱及其与临床特征和体重减轻效果的相关性。
结果
SG术前和术后共鉴定出85个差异表达的miRNA，而RYGB术前和术后共发现76个。这些miRNA的靶基因在SG和RYGB的基因本体富集分析中相似，京都基因与基因组百科全书中的富集分析主要与代谢途径相关。Hsa-miR-493-5p、hsa-miR-184和hsa-miR-3199在SG和RYGB中表现出相似的变化，前两者与临床特征相关。Hsa-miR-6729-5p、hsa-miR-4659b-5p和hsa-miR-2277-5p与SG的体重减轻效果相关，而hsa-miR-4662a-5p与RYGB的体重减轻效果相关。
结论
SG和RYGB手术后短期内的代谢改善和体重减轻可能与miRNA的变化有关，miRNA通过调节基因作用于多种生物学途径。此外，一些临床特征和miRNA与SG和RYGB手术后早期体重减轻的效果相关。
临床试验注册
ChiCTR2200058333。
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