ISREC - Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland.
Division of Breast Cancer Research, The Breast Cancer Now Toby Robins Research Centre, The Institute of Cancer Research, London, UK.
Methods Mol Biol. 2024;2811:101-112. doi: 10.1007/978-1-0716-3882-8_7.
Here, we describe a clinically relevant xenograft model of hormone receptor-positive breast cancer that maintains estrogen receptor (ER) status without the need for exogenous supplementation of hormones. The naturally low 17-β-estradiol levels in host mice recapitulate levels seen in post-menopausal women. By introducing breast cancer cells directly into their "natural" microenvironment of the milk ducts, these cells maintain hormone receptor status, model the clinical progression of the disease, and develop ER metastatic lesions or dormant micrometastatic lesions in the case of ER BC. With the use of GFP/RFP:Luc2 reporters, we can monitor in vivo tumour growth and conduct ex vivo metastases assays to evaluate dormant metastatic cell harboring organs. Upon recovery of metastatic cells from ER breast cancer models, downstream analyses can be conducted to assess the relationship between epithelial plasticity and metastatic dormancy.
在这里，我们描述了一种具有临床相关性的激素受体阳性乳腺癌异种移植模型，该模型无需外源性激素补充就能维持雌激素受体（ER）状态。宿主小鼠中天然低水平的 17-β-雌二醇水平再现了绝经后妇女的水平。通过将乳腺癌细胞直接引入其乳腺导管的“天然”微环境中，这些细胞维持激素受体状态，模拟疾病的临床进展，并在 ERBC 的情况下发展 ER 转移病灶或休眠微转移病灶。通过使用 GFP/RFP:Luc2 报告基因，我们可以监测体内肿瘤生长并进行离体转移实验，以评估携带休眠转移细胞的器官。从 ER 乳腺癌模型中回收转移细胞后，可以进行下游分析，以评估上皮可塑性与转移休眠之间的关系。
