[PM与O亚慢性联合暴露对大鼠鼻黏膜ATP含量及ATP酶活性的影响]
[Effects of PM and O sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa].
作者信息
Yan Tenglong, Xu Jiayu, Chen Tian, Yang Xin, Wang Weiwei, Zhou Shupei, Niu Piye, Jia Guang, Xia Jiao
机构信息
Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
Beijing Institute of Occupational Disease Prevention and Treatment, Beijing 100093, China.
出版信息
Beijing Da Xue Xue Bao Yi Xue Ban. 2024 Aug 18;56(4):687-692. doi: 10.19723/j.issn.1671-167X.2024.04.023.
OBJECTIVE
To evaluate the effects of fine particle matter (PM) and ozone (O) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats.
METHODS
Twenty male SD rats were divided into control group (=10) and exposure group (=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM and O in the exposure system were monitored, and a comprehensive assessment of PM and O in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na-K -ATPase and Ca -ATPase were detected by spectrophotometry. The test of two independent samples was used to compare the differences among the indicator groups.
RESULTS
From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group ( < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM concentration in the exposure group was (30.68±19.23) μg/m, and the maximum 8 h ozone concentration (O-8 h) was (82.45±35.81) μg/m. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na-K-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg ( < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups.
CONCLUSION
The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM and O.
目的
评估细颗粒物（PM）与臭氧（O₃）联合暴露对斯普拉格-道利（SD）大鼠鼻黏膜三磷酸腺苷（ATP）含量及ATP酶活性的影响。
方法
采用随机数字表法将20只雄性SD大鼠分为对照组（n = 10）和暴露组（n = 10）。大鼠分别在常规清洁环境和本团队建立的空气污染物暴露系统中饲养，并暴露208天。暴露期间，监测暴露系统中PM和O₃的浓度，通过自行测量与现场数据相结合的方式对暴露系统中的PM和O₃进行综合评估。在暴露第208天时，采集大鼠的心脏、肝脏、脾脏、肾脏、睾丸等主要器官及鼻黏膜组织。称取各器官重量并计算器官系数。采用生物发光法测定ATP总量，采用分光光度法检测Na⁺-K⁺-ATP酶和Ca²⁺-ATP酶活性。采用两独立样本t检验比较各指标组间差异。
结果
从第3周直至暴露结束，暴露组大鼠体重高于对照组（P < 0.05），两组间器官系数无显著差异。暴露组PM日均浓度为（30.68±19.23）μg/m³，最大8小时臭氧浓度（O₃-8h）为（82.45±35.81）μg/m³。暴露组大鼠鼻黏膜ATP的化学发光值（792.4±274.1）IU/L低于对照组（1126.8±218.1）IU/L。暴露组大鼠鼻黏膜Na⁺-K⁺-ATP酶活性（1.53±0.85）U/mg低于对照组（4.31±1.60）U/mg（P < 0.05）。对照组和暴露组鼻黏膜蛋白含量分别为（302.14±52.51）mg/L和（234.58±53.49）mg/L，Ca²⁺-ATP酶活性分别为（0.81±0.27）U/mg和（0.99±0.73）U/mg，两组间无显著差异。
结论
在PM与O₃亚慢性低浓度暴露下，大鼠鼻黏膜能量代谢能力下降。
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