State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Guangdong Province Key Laboratory of Display Material and Technology, Sun Yat-Sen University, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-Sen University, Shenzhen 518107, China.
Biosensors (Basel). 2024 Jul 2;14(7):328. doi: 10.3390/bios14070328.
Dopamine (DA), ascorbic acid (AA), and uric acid (UA) are crucial neurochemicals, and their abnormal levels are involved in various neurological disorders. While electrodes for their detection have been developed, achieving the sensitivity required for in vivo applications remains a challenge. In this study, we proposed a synthetic AuCd nanoenzyme (ACNE) that significantly enhanced the electrochemical performance of metal electrodes. ACNE-modified electrodes demonstrated a remarkable 10-fold reduction in impedance compared to silver microelectrodes. Furthermore, we validated their excellent electrocatalytic activity and sensitivity using five electrochemical detection methods, including cyclic voltammetry, differential pulse voltammetry, square-wave pulse voltammetry, normal pulse voltammetry, and linear scanning voltammetry. Importantly, the stability of gold microelectrodes (Au MEs) modified with ACNEs was significantly improved, exhibiting a 30-fold enhancement compared to Au MEs. This improved performance suggests that ACNE functionalization holds great promise for developing micro-biosensors with enhanced sensitivity and stability for detecting small molecules.
多巴胺（DA）、抗坏血酸（AA）和尿酸（UA）是至关重要的神经化学物质，它们的异常水平与各种神经紊乱有关。虽然已经开发出用于检测它们的电极，但实现体内应用所需的灵敏度仍然是一个挑战。在这项研究中，我们提出了一种合成的 AuCd 纳米酶（ACNE），它显著提高了金属电极的电化学性能。与银微电极相比，ACNE 修饰的电极的阻抗显著降低了 10 倍。此外，我们使用五种电化学检测方法验证了它们的优异的电催化活性和灵敏度，包括循环伏安法、差分脉冲伏安法、方波脉冲伏安法、正常脉冲伏安法和线性扫描伏安法。重要的是，ACNE 修饰的金微电极（Au MEs）的稳定性显著提高，与 Au MEs 相比，稳定性提高了 30 倍。这种性能的改善表明，ACNE 功能化在开发具有增强的灵敏度和稳定性的用于检测小分子的微生物传感器方面具有很大的潜力。
