Rhoden D L, Smith P B, Baker C N, Schable B
J Clin Microbiol. 1985 Dec;22(6):915-8. doi: 10.1128/jcm.22.6.915-918.1985.
A production model of the autoSCAN-4 system (American MicroScan, Inc., Mahwah, N.J.) was tested with not more than 11 strains each of 73 groups or species of gram-negative bacilli from various Centers for Disease Control culture collections. The strains included typical and atypical strains of enteric fermenters, nonenteric fermenters, and nonfermenters. The autoSCAN-4 system identified 95.3% of all 405 cultures accurately: 95.4% of 307 members of the family Enterobacteriaceae, 96.6% of 29 nonenteric fermenters, and 94.2% of 69 nonfermenters. Manual readings of the same trays provided essentially the same results, with a maximum change of only +1.6% identification accuracy of members of the Enterobacteriaceae. These data were obtained by all required additional tests, including serology and computer consultation when indicated. Only 19 of the cultures tested were misidentified. These were distributed randomly throughout the various groups and species except that Edwardsiella tarda was usually missed because of poor H2S reactions in the test medium. Of six Yersinia enterocolitica isolates, two were not identified. Only one nonenteric fermenter, a Pasteurella sp., and four nonfermenters (three Pseudomonas sp. and one Centers for Disease Control group Ve-2) were misidentified.
对autoSCAN-4系统（美国MicroScan公司，新泽西州马霍瓦）的一个生产型号进行了测试，使用了来自各疾病控制中心培养物保藏中心的73个革兰氏阴性杆菌组或种，每组不超过11株菌株。这些菌株包括肠道发酵菌、非肠道发酵菌和非发酵菌的典型及非典型菌株。autoSCAN-4系统准确鉴定了所有405份培养物中的95.3%：肠杆菌科307个成员中的95.4%，29个非肠道发酵菌中的96.6%，以及69个非发酵菌中的94.2%。对同一平板进行人工判读得到了基本相同的结果，肠杆菌科成员的鉴定准确率最大变化仅为+1.6%。这些数据是通过所有必需的额外检测获得的，包括血清学检测以及在需要时进行计算机咨询。所测试的培养物中只有19份被误鉴定。这些误鉴定随机分布在各个组和种中，不过迟缓爱德华氏菌通常因测试培养基中硫化氢反应不佳而被漏检。6株小肠结肠炎耶尔森菌分离株中有2株未被鉴定出来。只有1株非肠道发酵菌（一株巴斯德菌属细菌）和4株非发酵菌（3株假单胞菌属细菌和1株疾病控制中心Ve-2组细菌）被误鉴定。
