鲎试剂检测和家兔热原检测的灵敏度和特异性研究。
Studies on the sensitivity and specificity of the Limulus amebocyte lysate test and rabbit pyrogen assays.
作者信息
Devleeschouwer M J, Cornil M F, Dony J
出版信息
Appl Environ Microbiol. 1985 Dec;50(6):1509-11. doi: 10.1128/aem.50.6.1509-1511.1985.
Abstract
The sensitivity and specificity of the Limulus amebocyte lysate test and rabbit pyrogen assay were studied by means of artificially contaminated parenterals. Various gram-negative and gram-positive bacterial strains were used as was one strain of the yeast Candida albicans. The numbers of organisms needed to elicit positive responses in distilled water and normal saline were recorded and compared. The sensitivity and specificity of the Limulus amebocyte lysate assay for the detection of bacterial endotoxin from gram-negative bacteria were demonstrated. Variable results were recorded with gram-positive bacteria and Candida albicans.
摘要
通过人工污染的注射剂研究了鲎试剂检测法和家兔热原试验的灵敏度和特异性。使用了各种革兰氏阴性菌和革兰氏阳性菌菌株以及一株白色念珠菌酵母。记录并比较了在蒸馏水和生理盐水中引发阳性反应所需的微生物数量。证明了鲎试剂检测法检测革兰氏阴性菌内毒素的灵敏度和特异性。革兰氏阳性菌和白色念珠菌的检测结果不一。
相似文献
1
Studies on the sensitivity and specificity of the Limulus amebocyte lysate test and rabbit pyrogen assays.鲎试剂检测和家兔热原检测的灵敏度和特异性研究。
Appl Environ Microbiol. 1985 Dec;50(6):1509-11. doi: 10.1128/aem.50.6.1509-1511.1985.
2
3
[Application of the limulus amebocyte lysate test to measurement of endotoxin in therapeutic human plasma protein fraction. Comparison with the rabbit pyrogen test].鲎试剂检测法在治疗用人体血浆蛋白组分内毒素测定中的应用。与家兔热原试验的比较
Yakugaku Zasshi. 1992 Aug;112(8):551-6. doi: 10.1248/yakushi1947.112.8_551.
4
Comparison of cultured human mononuclear cells, Limulus amebocyte lysate and rabbits in the detection of pyrogens.人外周血单个核细胞、鲎试剂与家兔用于检测热原质的比较。
J Clin Pharm Ther. 1990 Dec;15(6):425-33. doi: 10.1111/j.1365-2710.1990.tb00407.x.
5
A highly sensitive pyrogen test for antibiotics I: detection of trace amounts of endotoxin in injectable sodium ampicillin preparations.抗生素的一种高灵敏度热原检测方法I：注射用氨苄西林钠制剂中痕量内毒素的检测
J Pharm Sci. 1983 Jul;72(7):739-42. doi: 10.1002/jps.2600720706.
6
Comparison of the pyrogen tests in rabbits and with limulus lysate.家兔热原试验与鲎试剂热原试验的比较。
Dev Biol Stand. 1977;34:27-36.
7
A comparison between the Limulus amebocyte lysate test and the rabbit pyrogen test.鲎试剂检测法与家兔热原检测法的比较。
J Pharm Belg. 1979 May-Jun;34(3):142-4.
8
[Comparison of the Limulus test with the pyrogen test in rabbits].鲎试剂检测法与家兔热原检测法的比较
Acta Biol Med Ger. 1980;39(2-3):277-80.
9
Applications and advantages of the Limulus amebocyte lysate (LAL) pyrogen test for parenteral injectable products.鲎试剂热原检测法在注射用药品中的应用及优势。
Prog Clin Biol Res. 1979;29:387-402.
10
Human monocytoid cell lines as indicators of endotoxin: comparison with rabbit pyrogen and Limulus amoebocyte lysate assay.人单核细胞样细胞系作为内毒素指标：与兔热原和鲎试剂检测法的比较
J Immunol Methods. 1997 Sep 24;207(2):135-45. doi: 10.1016/s0022-1759(97)00112-9.
引用本文的文献
1
Development of a novel bacterial production system for recombinant bioactive proteins completely free from endotoxin contamination.开发一种全新的细菌生产系统，用于生产完全无内毒素污染的重组生物活性蛋白。
PNAS Nexus. 2024 Aug 7;3(8):pgae328. doi: 10.1093/pnasnexus/pgae328. eCollection 2024 Aug.
2
LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface.LptE 结合并改变 LPS 的物理状态，以催化其在细胞表面的组装。
Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9467-72. doi: 10.1073/pnas.1402746111. Epub 2014 Jun 17.
3
Prognostic value of endotoxemia in patients with Gram-negative bacteremia is bacterial species dependent.内毒素血症对革兰氏阴性菌血症患者的预后价值取决于细菌种类。
J Innate Immun. 2013;5(6):555-64. doi: 10.1159/000347172. Epub 2013 Apr 13.
4
Endotoxemia and mortality prediction in ICU and other settings: underlying risk and co-detection of gram negative bacteremia are confounders.重症监护病房及其他环境中的内毒素血症与死亡率预测：潜在风险以及革兰氏阴性菌血症的联合检测为混杂因素。
Crit Care. 2012 Aug 7;16(4):R148. doi: 10.1186/cc11462.
5
Does gram-negative bacteraemia occur without endotoxaemia? A meta-analysis using hierarchical summary ROC curves.革兰氏阴性菌血症是否会发生而没有内毒素血症？使用分层汇总 ROC 曲线的荟萃分析。
Eur J Clin Microbiol Infect Dis. 2010 Feb;29(2):207-15. doi: 10.1007/s10096-009-0841-2. Epub 2009 Nov 29.
6
Diagnosis of endotoxemia with gram-negative bacteremia is bacterial species dependent: a meta-analysis of clinical studies.革兰氏阴性菌菌血症患者的内毒素血症诊断依赖于细菌种类：临床研究的荟萃分析。
J Clin Microbiol. 2009 Dec;47(12):3826-31. doi: 10.1128/JCM.01189-09. Epub 2009 Sep 30.
7
[Endotoxins. Pathogenetic meaning of sepsis].[内毒素。脓毒症的发病机制意义]
Anaesthesist. 2003 Dec;52 Suppl 1:S7-S13. doi: 10.1007/s00101-003-0587-7.
8
Endotoxemia: methods of detection and clinical correlates.内毒素血症：检测方法及临床关联
Clin Microbiol Rev. 1995 Apr;8(2):268-92. doi: 10.1128/CMR.8.2.268.
本文引用的文献
1
The Pyrogenicity of Bacterial Contaminants Found in Biologic Products.生物制品中发现的细菌污染物的致热原性。
J Bacteriol. 1945 Oct;50(4):397-411.
2
Relative potencies of four reference endotoxin standards as measured by the Limulus amoebocyte lysate and USP rabbit pyrogen tests.通过鲎试剂法和美国药典家兔热原试验测定的四种参考内毒素标准品的相对效价。
Appl Environ Microbiol. 1980 Dec;40(6):1148-51. doi: 10.1128/aem.40.6.1148-1151.1980.
3
Effect of nonsterility on the activity of Limulus amebocyte lysate.非无菌状态对鲎试剂活性的影响。
J Parenter Sci Technol. 1982 Sep-Oct;36(5):196-8.
4
A technique for quantitative measurement of endotoxin in human plasma.一种定量测量人血浆中内毒素的技术。
Proc Soc Exp Biol Med. 1971 May;137(1):334-40. doi: 10.3181/00379727-137-35572.
5
Investigations on the specificity of the Limulus test for the detection of endotoxin.鲎试剂检测内毒素特异性的研究。
Appl Microbiol. 1974 Nov;28(5):867-71. doi: 10.1128/am.28.5.867-871.1974.
6
[Contribution to the diagnosis of shock: demonstration of endotoxin and mucopeptide by the Limulus polyphemus-lysate test (author's transl)].[对休克诊断的贡献：用鲎试剂试验检测内毒素和粘肽（作者译）]
Klin Wochenschr. 1974 Feb 15;52(4):175-8. doi: 10.1007/BF01614393.
7
Rapid detection of contaminated intravenous fluids using the Limulus in vitro endotoxin assay.利用鲎试剂体外内毒素测定法快速检测受污染的静脉输液。
Appl Microbiol. 1973 Oct;26(4):521-4. doi: 10.1128/am.26.4.521-524.1973.
8
Measurement of bound and free endotoxin by the Limulus assay.用鲎试剂法测定结合内毒素和游离内毒素。
Proc Soc Exp Biol Med. 1974 Sep;146(4):1024-31. doi: 10.3181/00379727-146-38240.
9
Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay.鲎试剂用于内毒素检测的制备、灵敏度和特异性。
Appl Microbiol. 1973 Jul;26(1):43-8. doi: 10.1128/am.26.1.43-48.1973.
Zotero 插件