Division of Gastroenterology and Hepatology, Department of Internal Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Division of Research and Development for Minimally Invasive Treatment Cancer Center, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Sci Rep. 2024 Aug 12;14(1):18705. doi: 10.1038/s41598-024-69820-7.
The incidence of duodenal tumors (DTs) is increasing. However, the mechanisms underlying its development remain unclear. Environmental factors, including the microbiome and bile acids (BAs), are believed to influence tumor development. Therefore, we conducted a single-center, prospective, observational study to investigate the potential differences between patients with DTs and healthy controls (HCs) based on these factors. In addition, the BAs in the duodenal fluid were measured using liquid chromatography-tandem mass spectrometry. We recruited 41 patients and performed 16S rRNA-seq. There was no difference in the observed ASVs or PCoA plot of Bray-Curtis dissimilarity between the DTs and HCs. The lithocholic acid concentration was significantly lower in the DT group than in the control group. The ratio of CDCA to LCA was significantly higher in patients with DTs. No significant differences in microbiota were observed between DTs and HCs. In patients with DTs, the lithocholic acid concentration in duodenal was significantly lower than in HCs.
十二指肠肿瘤 (DTs) 的发病率正在上升。然而，其发展的机制尚不清楚。环境因素，包括微生物组和胆汁酸 (BAs)，被认为会影响肿瘤的发展。因此，我们进行了一项单中心、前瞻性、观察性研究，基于这些因素，研究 DTs 患者和健康对照 (HCs) 之间的潜在差异。此外，使用液相色谱-串联质谱法测量十二指肠液中的胆汁酸。我们招募了 41 名患者并进行了 16S rRNA-seq。在 DTs 和 HCs 之间，观察到的 ASVs 或 Bray-Curtis 不相似性的 PCoA 图谱没有差异。DT 组的石胆酸浓度明显低于对照组。DTs 患者的 CDCA 与 LCA 比值明显更高。DTs 和 HCs 之间的微生物群没有观察到显著差异。在 DTs 患者中，十二指肠的石胆酸浓度明显低于 HCs。
