Guangdong Provincial Key Laboratory of Advanced Biomaterials, Shenzhen Key Laboratory of Smart Healthcare Engineering, Department of Biomedical Engineering, Southern University of Science and Technology, No 1088, Xueyuan Road, Nanshan District, Shenzhen, Guangdong 518055, People's Republic of China.
Department of Electronic and Electrical Engineering, Southern University of Science and Technology, No 1088, Xueyuan Road, Nanshan District, Shenzhen, Guangdong 518055, People's Republic of China.
ACS Nano. 2023 Feb 14;17(3):2840-2850. doi: 10.1021/acsnano.2c11241. Epub 2023 Feb 2.
DNA as an exceptional data storage medium offers high information density. However, DNA storage requires specialized equipment and tightly controlled environments for storage. Fast encapsulation within minutes for enhanced DNA stability to do away with specialized equipment and fast DNA extraction remain a challenge. Here, we report a DNA microlibrary that can be encapsulated by metal-organic frameworks (MOFs) within 10 min and extracted (5 min) in a single microfluidic chip for automated and integrated DNA-based data storage. The DNA microlibrary@MOFs enhances the stability of data-encoded DNA against harsh environments. The encoded information can be read out perfectly after accelerated aging, equivalent to being readable after 10 years of storage at 25 °C, 50% relative humidity, and 10 000 lx sunlight radiation. Moreover, the library enables fast retrieval of target data via flow cytometry and can be reproduced after each access.
DNA 作为一种特殊的数据存储介质，具有很高的信息密度。然而，DNA 存储需要专门的设备和严格控制的环境来进行存储。快速封装以提高 DNA 稳定性，避免使用专门设备和快速 DNA 提取仍然是一个挑战。在这里，我们报告了一种 DNA 微文库，可以在 10 分钟内被金属有机框架（MOFs）封装，并在单个微流控芯片中进行（5 分钟）提取，用于自动化和集成的基于 DNA 的数据存储。DNA 微文库@MOFs 增强了数据编码 DNA 对恶劣环境的稳定性。经过加速老化后，编码信息可以完美读取，相当于在 25°C、50%相对湿度和 10000 lx 阳光辐射下储存 10 年后仍可读取。此外，该文库可以通过流式细胞术快速检索目标数据，并且在每次访问后都可以重现。
