Kadurugamuwa J L, Anwar H, Brown M R, Zak O
Antimicrob Agents Chemother. 1985 Aug;28(2):195-9. doi: 10.1128/AAC.28.2.195.
It recently has been reported by us that cephalosporins, at a concentration below that influencing growth rate, reduced the production of enterochelin and capsule formation of iron-depleted Klebsiella pneumoniae. We now report on the antigenicity of the outer membrane components and surface-exposed protein antigens of iron-depleted cells grown in the presence or absence of cephalosporins. All major outer membrane proteins, including iron-regulated membrane proteins, were immunogenic. Encapsulated K. pneumoniae grown in antibiotic-free media had three protein antigens (60, 35.5, and 32.5 kilodaltons) exposed on the surface that were accessible to antibodies. Growth of the same cultures in the presence of subinhibitory concentrations of cephalosporins resulted in the exposure of a greater number of protein antigenic determinants, including iron-regulated membrane proteins, which become readily accessible to antibodies. It was also found that immunoblotting was generally more sensitive than conventional staining of the acrylamide gel with Coomassie blue in the detection of proteins.
我们最近报道，头孢菌素在低于影响生长速率的浓度下，可减少肠杆菌素的产生，并抑制缺铁肺炎克雷伯菌的荚膜形成。我们现在报告在有或没有头孢菌素存在的情况下生长的缺铁细胞的外膜成分和表面暴露蛋白抗原的抗原性。所有主要的外膜蛋白，包括铁调节膜蛋白，都具有免疫原性。在无抗生素培养基中生长的包膜肺炎克雷伯菌有三种表面暴露的蛋白抗原（60、35.5和32.5千道尔顿），抗体可以识别。在亚抑制浓度的头孢菌素存在下培养相同的菌株，会导致更多数量的蛋白抗原决定簇暴露，包括铁调节膜蛋白，这些蛋白变得易于被抗体识别。还发现，在蛋白质检测中，免疫印迹通常比用考马斯亮蓝对丙烯酰胺凝胶进行传统染色更灵敏。
