Surasa Wilasinee, Pientong Chamsai, Ekalaksananan Tipaya, Overgaard Hans Jorgen, Aromseree Sirinart, Phanthanawiboon Supranee
Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
HPV&EBV and Carcinogenesis, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Vet World. 2024 Jul;17(7):1555-1561. doi: 10.14202/vetworld.2024.1555-1561. Epub 2024 Jul 21.
Some mosquitoes are competent vectors for Japanese encephalitis virus (JEV) and Zika virus (ZIKV), which cause public health problems worldwide, especially in South-east Asia. Xenosurveillance of mosquitoes remains limited compared with other common mosquito-borne diseases. This study aimed to identify JEV and ZIKV in field-caught mosquitoes collected from Ubon Ratchathani province.
We investigated the presence of JEV and ZIKV in mosquitoes from two districts in Ubon Ratchathani province, Thailand, and examined their role in viral interepidemic circulation. Female mosquitoes (5,587) were collected using a mechanical aspirator from indoors and outdoors. The consensus sequences of the E and NS1 genes of JEV and the E gene of ZIKV were identified using real-time reverse transcription-polymerase chain reaction.
From 335 sample pools that contain a total of 5587 adult female Culex mosquitoes collected from Don Yung, Mueang district (4,406) and Phon Duan, Det Udom district (1,181), none of the collected mosquitoes tested positive for either JEV or ZIKV.
This study did not find JEV and ZIKV in mosquitoes collected from the area of collection, which may be due to the low circulating amount of the virus in the vectors in the area, making it undetectable, or it may be because mosquitoes are not suitable vector for the virus being tested. However, further xenosurveillance study of JEV and ZIKV in mosquito is suggested to prepare for the next outbreak.
一些蚊子是日本脑炎病毒（JEV）和寨卡病毒（ZIKV）的有效传播媒介，这两种病毒在全球范围内引发公共卫生问题，尤其是在东南亚地区。与其他常见的蚊媒疾病相比，对蚊子的异体监测仍然有限。本研究旨在鉴定从乌汶叻差他尼府采集的野外捕获蚊子中的JEV和ZIKV。
我们调查了泰国乌汶叻差他尼府两个地区蚊子中JEV和ZIKV的存在情况，并研究了它们在病毒间期传播中的作用。使用机械吸气器从室内和室外收集雌性蚊子（5587只）。通过实时逆转录聚合酶链反应鉴定JEV的E和NS1基因以及ZIKV的E基因的共有序列。
从总共包含5587只成年雌性库蚊的335个样本组中收集了蚊子，这些蚊子分别来自孟区的Don Yung（4406只）和德乌东府的Phon Duan（1181只），所收集的蚊子中没有一只JEV或ZIKV检测呈阳性。
本研究在采集区域的蚊子中未发现JEV和ZIKV，这可能是由于该区域媒介中病毒的循环量低，导致无法检测到，或者可能是因为蚊子不是所检测病毒的合适媒介。然而，建议对蚊子中的JEV和ZIKV进行进一步的异体监测研究，为下一次疫情爆发做好准备。
