Gao Yang, Ma Qingming
School of Pharmacy Qingdao University Qingdao China.
Key Laboratory of Functional Polymer Materials of Ministry of Education State Key Laboratory of Medicinal Chemical Biology and Institute of Polymer Chemistry College of Chemistry Nankai University Tianjin China.
Smart Med. 2022 Dec 16;1(1):e20220012. doi: 10.1002/SMMD.20220012. eCollection 2022 Dec.
The overuse of antibiotics for treating bacterial infection has caused severe bacterial resistance and become a public health threat worldwide. It is desired to develop novel antibiotic delivery systems as efficient antibacterial strategies for promoting anti-infective therapy. Herein, the AgNPs-loaded -[(2-hydroxy-3-trimethyl ammonium) propyl] chitosan (HTCC)/hyaluronic acid (HA) porous microspheres (HHPMs) by microfluidics have been developed as novel bacterial infection microenvironment (IME)-responsive antibiotic delivery systems for promoting antimicrobial therapy. The release of AgNPs can respond explicitly to the IME with acidic pH values and relatively high hyaluronidase concentration. The unique porous structures of HHPMs can effectively facilitate the capture and enrichment of bacteria, thus exerting synergistic antibacterial effects, which can be more efficient in instant bacteria inhibiting and killing. The excellent biocompatibility of HHPMs is revealed by investigating their hemolytic activity and cytotoxicity. In vivo assays demonstrate that the fabricated AgNPs-loaded HHPMs can effectively resist bacterial infection and promote wound healing and tissue regeneration at infected wound sites by inhibition of the bacterial survival. This work indicates that fabricated HHPMs are ideal bacterial infection microenvironment-responsive materials for antibiotic delivery and show great promises for promoting anti-infective therapy in clinics.
抗生素在治疗细菌感染方面的过度使用已导致严重的细菌耐药性，并成为全球公共卫生威胁。人们期望开发新型抗生素递送系统作为有效的抗菌策略，以促进抗感染治疗。在此，通过微流控技术制备了负载银纳米颗粒的-[(2-羟基-3-三甲基铵)丙基]壳聚糖(HTCC)/透明质酸(HA)多孔微球(HHPMs)，作为新型细菌感染微环境(IME)响应性抗生素递送系统，以促进抗菌治疗。银纳米颗粒的释放可以对具有酸性pH值和相对较高透明质酸酶浓度的IME做出明确响应。HHPMs独特的多孔结构可以有效地促进细菌的捕获和富集，从而发挥协同抗菌作用，在即时抑制和杀灭细菌方面更有效。通过研究HHPMs的溶血活性和细胞毒性，揭示了其优异的生物相容性。体内实验表明，制备的负载银纳米颗粒的HHPMs可以有效抵抗细菌感染，并通过抑制细菌存活促进感染伤口部位的伤口愈合和组织再生。这项工作表明，制备的HHPMs是理想的细菌感染微环境响应性抗生素递送材料，在促进临床抗感染治疗方面具有巨大潜力。
