Heuchel Alisa, Emblem Åse, Jørgensen Tor Erik, Moum Truls, Johansen Steinar Daae
Genomic Division, Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
Abisko Scientific Research Station, Swedish Polar Research Secretariat, SE-981 07 Abisko, Sweden.
Curr Issues Mol Biol. 2024 Jul 27;46(8):8104-8110. doi: 10.3390/cimb46080479.
We sequenced and analyzed the complete mitogenome of a Norwegian isolate of the octocoral using the Ion Torrent sequencing technology. The 18,790 bp circular mitochondrial genome was found to harbor the same set of 17 genes, which encode 14 protein subunits, two structural ribosomal RNAs and one tRNA, as reported in other octocorals. In addition, we detected a new tRNA-like gene sequence nested within the MutS protein coding region. This putative tRNA gene feature appears to be conserved among the octocorals but has not been reported previously. The mitogenome was also shown to harbor an optional gene (ORFA) that encodes a putative protein of 191 amino acids with unknown function. A mitogenome-based phylogenetic analysis, presented as a maximum likelihood tree, showed that clustered with high statistical confidence with two other species endemic to the Mediterranean Sea and the Southeast Pacific Ocean.
我们使用离子激流测序技术对一种挪威八放珊瑚分离株的完整线粒体基因组进行了测序和分析。发现这个18,790 bp的环状线粒体基因组含有与其他八放珊瑚中报道的相同的17个基因组合，这些基因编码14个蛋白质亚基、两个结构性核糖体RNA和一个tRNA。此外，我们在MutS蛋白编码区域内检测到一个新的类似tRNA的基因序列。这种假定的tRNA基因特征似乎在八放珊瑚中是保守的，但此前尚未见报道。该线粒体基因组还显示含有一个可选基因（ORFA），它编码一个功能未知的191个氨基酸的假定蛋白质。基于线粒体基因组的系统发育分析以最大似然树呈现，结果显示该分离株与另外两种地中海和东南太平洋特有的物种以高统计学置信度聚类在一起。
