Antimicrobial Activity of Olive Leaf Extract to Oral Isolates.

OBJECTIVES

The aim of this study was to determine the antifungal activity of olive leaf extract (OLE) and the synergistic effect of standard antifungal therapy and OLE against clinical oral species' isolates.

MATERIALS AND METHOD

The susceptibility of 60 clinical isolates of the species (36 , 16 , 5 and 3 ) was tested with four concentrations of OLE (60 µg/µL, 120 µg/µL, 240 µg/µL and 333 µg/µL) and the synergistic effect of standard antifungal therapy and OLE (miconazole (MIC) + 333 µg/µL OLE and nystatin (NYS) + 333 µg/µL OLE). The antimicrobial activity was tested using the disk diffusion method.

RESULTS

All concentrations (60 µg/µL, 120 µg/µL, 240 µg/µL and 333 µg/µL) of OLE showed a statistically significant effect on all species compared to the control (DMSO) except for the lowest concentration (60 µg/µL) tested on . There was a dose-dependent effect of OLE on tested samples. Concentrations of 240 µg/µL and 333 µg/µL showed statistically significant higher antifungal activity compared to the lowest concentration of 60 µg/µL. No statistically significant synergistic effect of OLE and standard antifungal therapy was found compared with standard therapy alone.

CONCLUSIONS

The results of this study present the significant antimicrobial effect of OLE against all tested species except for the lowest concentration on . Increasing the concentration of OLE also increases its effect on species. This indicates the possible potential effect of OLE in the treatment of -related oral diseases.