Kinkela Devčić Maja, Pasković Igor, Kovač Zoran, Knežević Petra Tariba, Morelato Luka, Glažar Irena, Simonić-Kocijan Sunčana
Department of Oral Surgery, Faculty of Dental Medicine, University of Rijeka, Kresimirova 40/42, 51000 Rijeka, Croatia.
Department of Agriculture and Nutrition, Institute of Agriculture and Tourism, K. Huguesa 8, 52440 Porec, Croatia.
Microorganisms. 2024 Aug 21;12(8):1726. doi: 10.3390/microorganisms12081726.
The aim of this study was to determine the antifungal activity of olive leaf extract (OLE) and the synergistic effect of standard antifungal therapy and OLE against clinical oral species' isolates.
The susceptibility of 60 clinical isolates of the species (36 , 16 , 5 and 3 ) was tested with four concentrations of OLE (60 µg/µL, 120 µg/µL, 240 µg/µL and 333 µg/µL) and the synergistic effect of standard antifungal therapy and OLE (miconazole (MIC) + 333 µg/µL OLE and nystatin (NYS) + 333 µg/µL OLE). The antimicrobial activity was tested using the disk diffusion method.
All concentrations (60 µg/µL, 120 µg/µL, 240 µg/µL and 333 µg/µL) of OLE showed a statistically significant effect on all species compared to the control (DMSO) except for the lowest concentration (60 µg/µL) tested on . There was a dose-dependent effect of OLE on tested samples. Concentrations of 240 µg/µL and 333 µg/µL showed statistically significant higher antifungal activity compared to the lowest concentration of 60 µg/µL. No statistically significant synergistic effect of OLE and standard antifungal therapy was found compared with standard therapy alone.
The results of this study present the significant antimicrobial effect of OLE against all tested species except for the lowest concentration on . Increasing the concentration of OLE also increases its effect on species. This indicates the possible potential effect of OLE in the treatment of -related oral diseases.
本研究旨在确定橄榄叶提取物（OLE）的抗真菌活性，以及标准抗真菌疗法与OLE联合对临床口腔分离菌株的协同作用。
使用四种浓度的OLE（60μg/μL、120μg/μL、240μg/μL和333μg/μL）测试60株临床分离菌株（36株 、16株 、5株 和3株 ）的敏感性，以及标准抗真菌疗法与OLE（咪康唑（MIC）+333μg/μL OLE和制霉菌素（NYS）+333μg/μL OLE）的协同作用。采用纸片扩散法测试抗菌活性。
与对照（二甲基亚砜）相比，除了对 测试的最低浓度（60μg/μL）外，OLE的所有浓度（60μg/μL、120μg/μL、240μg/μL和333μg/μL）对所有 菌株均显示出统计学上的显著效果。OLE对测试样品有剂量依赖性作用。与最低浓度60μg/μL相比，240μg/μL和333μg/μL浓度显示出统计学上显著更高的抗真菌活性。与单独的标准疗法相比，未发现OLE与标准抗真菌疗法有统计学上的显著协同作用。
本研究结果表明，除了对 测试的最低浓度外，OLE对所有测试的 菌株均有显著的抗菌作用。增加OLE的浓度也会增强其对 菌株的作用。这表明OLE在治疗与 相关的口腔疾病中可能具有潜在作用。
