Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, College of Animal Science and Technology, Guangxi University, Nanning 530003, China.
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China.
Viruses. 2024 Aug 21;16(8):1338. doi: 10.3390/v16081338.
Typhimurium ( Typhimurium) contamination poses a significant challenge to breeder egg hatchability and chick health, necessitating the exploration of alternative disinfection methods. This study investigates the potential of phage vB_SPuM_SP02 (SP02) as a novel disinfectant for breeder eggs contaminated with Typhimurium SM022. Phage SP02 was isolated from poultry farm effluent and characterized for morphology, biological properties, and genome properties. Experimental groups of specific pathogen-free (SPF) eggs were treated with and phage SP02, and efficacy was assessed through hatching rates, chick survival, weight, load, immune organ indices, and intestinal flora. Phage treatment effectively eradicated contamination on eggshells within 12 h, resulting in increased hatching and survival rates compared to controls. Furthermore, phage treatment mitigated weight loss and tissue load in chicks without causing immune organ damage while reducing spp. abundance in the intestinal tract. This study demonstrates the potential of phage SP02 as an eco-friendly and efficient disinfectant for . Typhimurium-contaminated breeder eggs, offering promising prospects for practical application in poultry production.
肠炎沙门氏菌（Typhimurium）污染对种蛋孵化率和雏鸡健康构成重大挑战，因此需要探索替代消毒方法。本研究探讨了噬菌体 vB_SPuM_SP02（SP02）作为一种新型消毒剂，用于消毒受肠炎沙门氏菌 SM022 污染的种蛋。噬菌体 SP02 从禽场污水中分离出来，并对其形态、生物学特性和基因组特性进行了研究。实验中，使用噬菌体 SP02 处理特定病原体（SPF）种蛋，通过孵化率、雏鸡存活率、体重、负荷、免疫器官指数和肠道菌群评估其效果。噬菌体处理可在 12 小时内有效清除蛋壳上的污染，与对照组相比，孵化率和存活率均有所提高。此外，噬菌体处理减轻了雏鸡的体重减轻和组织负荷，而不会造成免疫器官损伤，同时减少了肠道中的 spp. 丰度。本研究表明，噬菌体 SP02 具有作为一种环保、高效消毒剂的潜力，可用于消毒受肠炎沙门氏菌污染的种蛋，为家禽生产中的实际应用提供了有前景的选择。
