α-氨基己二酸作为酿酒酵母突变体的主要氮源。
alpha-Aminoadipate as a primary nitrogen source for Saccharomyces cerevisiae mutants.
作者信息
Zaret K S, Sherman F
出版信息
J Bacteriol. 1985 May;162(2):579-83. doi: 10.1128/jb.162.2.579-583.1985.
Abstract
In contrast to wild-type strains of the yeast Saccharomyces cerevisiae, lys2 and lys5 mutants are able to utilize alpha-aminoadipate as a primary source of nitrogen. Chattoo et al. (B. B. Chattoo, F. Sherman, D. A. Azubalis, T. A. Fjellstedt, D. Mehnert, and M. Ogur, Genetics 93:51-65, 1979) relied on this difference in the effective utilization of alpha-aminoadipate to develop a procedure for directly selecting lys2 and lys5 mutants. In this study we used a range of mutant strains and various media to determine why normal strains are unable to utilize alpha-aminoadipate as a nitrogen source. Our results demonstrate that the anabolism of high levels of alpha-aminoadipate through the biosynthetic pathway of lysine results in the accumulation of a toxic intermediate and, furthermore, that lys2 and lys5 mutants contain blocks leading to the formation of this intermediate.
摘要
与酿酒酵母的野生型菌株相比，lys2和lys5突变体能够利用α-氨基己二酸作为主要氮源。Chattoo等人（B. B. Chattoo、F. Sherman、D. A. Azubalis、T. A. Fjellstedt、D. Mehnert和M. Ogur，《遗传学》93:51 - 65，1979）利用α-氨基己二酸有效利用方面的这种差异，开发了一种直接筛选lys2和lys5突变体的方法。在本研究中，我们使用了一系列突变菌株和各种培养基，以确定正常菌株无法利用α-氨基己二酸作为氮源的原因。我们的结果表明，通过赖氨酸生物合成途径进行的高水平α-氨基己二酸的合成代谢会导致一种有毒中间体的积累，此外，lys2和lys5突变体含有导致该中间体形成的阻断。
相似文献
1
alpha-Aminoadipate as a primary nitrogen source for Saccharomyces cerevisiae mutants.α-氨基己二酸作为酿酒酵母突变体的主要氮源。
J Bacteriol. 1985 May;162(2):579-83. doi: 10.1128/jb.162.2.579-583.1985.
2
alpha-Aminoadipate pathway for the biosynthesis of lysine in lower eukaryotes.低等真核生物中赖氨酸生物合成的α-氨基己二酸途径。
Crit Rev Microbiol. 1985;12(2):131-51. doi: 10.3109/10408418509104427.
3
Selection of lys2 Mutants of the Yeast SACCHAROMYCES CEREVISIAE by the Utilization of alpha-AMINOADIPATE.利用 α-氨基己二酸筛选酵母 SACCHAROMYCES CEREVISIAE 的 lys2 突变株。
Genetics. 1979 Sep;93(1):51-65. doi: 10.1093/genetics/93.1.51.
4
Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5.酿酒酵母中的赖氨酸生物合成：α-氨基己二酸还原酶（Lys2）的机制涉及由Lys5进行的翻译后磷酸泛酰巯基乙胺化。
Biochemistry. 1999 May 11;38(19):6171-7. doi: 10.1021/bi9829940.
5
Properties of revertants of lys2 and lys5 mutants as well as alpha-aminoadipate-semialdehyde dehydrogenase from Saccharomyces cerevisiae.酿酒酵母lys2和lys5突变体回复突变株以及α-氨基己二酸半醛脱氢酶的特性
Biochem Biophys Res Commun. 1989 May 30;161(1):182-6. doi: 10.1016/0006-291x(89)91578-7.
6
Growth inhibition by alpha-aminoadipate and reversal of the effect by specific amino acid supplements in Saccharomyces cerevisiae.α-氨基己二酸对酿酒酵母的生长抑制作用以及特定氨基酸补充剂对该作用的逆转
J Bacteriol. 1982 Nov;152(2):874-9. doi: 10.1128/jb.152.2.874-879.1982.
7
Use of alpha-aminoadipate and lysine as sole nitrogen source by Schizosaccharomyces pombe and selected pathogenic fungi.粟酒裂殖酵母和部分致病真菌将α-氨基己二酸和赖氨酸用作唯一氮源的情况。
J Basic Microbiol. 1991;31(2):149-56. doi: 10.1002/jobm.3620310215.
8
Molecular analysis of the LYS2 gene of Candida albicans: homology to peptide antibiotic synthetases and the regulation of the alpha-aminoadipate reductase.白色念珠菌LYS2基因的分子分析：与肽抗生素合成酶的同源性及α-氨基己二酸还原酶的调控
Curr Genet. 1998 Apr;33(4):268-75. doi: 10.1007/s002940050336.
9
LYS2 gene and its mutation in Kluyveromyces lactis.乳酸克鲁维酵母中的LYS2基因及其突变
Yeast. 2003 Oct 30;20(14):1171-5. doi: 10.1002/yea.1030.
10
Physical and biochemical characterization of the cloned LYS5 gene required for alpha-aminoadipate reductase activity in the lysine biosynthetic pathway of Saccharomyces cerevisiae.酿酒酵母赖氨酸生物合成途径中α-氨基己二酸还原酶活性所需的克隆LYS5基因的物理和生化特性
Curr Genet. 1992 Jan;21(1):13-6. doi: 10.1007/BF00318647.
引用本文的文献
1
Understanding brewing trait inheritance in Lager yeast hybrids.了解拉格酵母杂交种中的酿造特性遗传。
mSystems. 2024 Dec 17;9(12):e0076224. doi: 10.1128/msystems.00762-24. Epub 2024 Nov 12.
2
Generation of intra- and interspecific Saccharomyces hybrids with improved oenological and aromatic properties.生成具有改良酿造和芳香特性的种内和种间酿酒酵母杂种。
Microb Biotechnol. 2022 Aug;15(8):2266-2280. doi: 10.1111/1751-7915.14068. Epub 2022 Apr 29.
3
Packing a punch: understanding how flavours are produced in lager fermentations.重拳出击：了解拉格发酵中风味的产生。
FEMS Yeast Res. 2021 Jul 24;21(5). doi: 10.1093/femsyr/foab040.
4
Industrially Applicable Lager Yeast Hybrids with a Unique Genomic Architecture: Creation and Characterization.具有独特基因组结构的工业适用啤酒酵母杂种：创建与特性。
Appl Environ Microbiol. 2021 Jan 15;87(3). doi: 10.1128/AEM.02434-20.
5
Differential Contribution of the Parental Genomes to a × Hybrid, Inferred by Phenomic, Genomic, and Transcriptomic Analyses, at Different Industrial Stress Conditions.在不同工业应激条件下，通过表型组学、基因组学和转录组学分析推断亲本基因组对×杂交种的差异贡献。
Front Bioeng Biotechnol. 2020 Mar 3;8:129. doi: 10.3389/fbioe.2020.00129. eCollection 2020.
6
Derailing the aspartate pathway of Mycobacterium tuberculosis to eradicate persistent infection.破坏结核分枝杆菌天冬氨酸途径以根除持续性感染。
Nat Commun. 2019 Sep 16;10(1):4215. doi: 10.1038/s41467-019-12224-3.
7
Improving the Cryotolerance of Wine Yeast by Interspecific Hybridization in the Genus .通过属间杂交提高酿酒酵母的耐冻性
Front Microbiol. 2019 Jan 8;9:3232. doi: 10.3389/fmicb.2018.03232. eCollection 2018.
8
Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.DNA重组与修复研究指南：DNA修复途径的细胞分析
Microb Cell. 2019 Jan 7;6(1):1-64. doi: 10.15698/mic2019.01.664.
9
Alternative Saccharomyces interspecies hybrid combinations and their potential for low-temperature wort fermentation.酿酒酵母种间杂交组合及其低温麦汁发酵潜力
Yeast. 2018 Jan;35(1):113-127. doi: 10.1002/yea.3246. Epub 2017 Aug 30.
10
Improved cider fermentation performance and quality with newly generated Saccharomyces cerevisiae × Saccharomyces eubayanus hybrids.利用新产生的酿酒酵母×真贝氏酿酒酵母杂种提高苹果酒发酵性能和品质。
J Ind Microbiol Biotechnol. 2017 Aug;44(8):1203-1213. doi: 10.1007/s10295-017-1947-7. Epub 2017 Apr 27.
本文引用的文献
1
Selection of lys2 Mutants of the Yeast SACCHAROMYCES CEREVISIAE by the Utilization of alpha-AMINOADIPATE.利用 α-氨基己二酸筛选酵母 SACCHAROMYCES CEREVISIAE 的 lys2 突变株。
Genetics. 1979 Sep;93(1):51-65. doi: 10.1093/genetics/93.1.51.
2
HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, II. GALACTOSE-INDUCED SENSITIVITY.大肠杆菌突变体中半乳糖代谢的遗传性缺陷，II. 半乳糖诱导的敏感性
Proc Natl Acad Sci U S A. 1959 Dec;45(12):1786-91. doi: 10.1073/pnas.45.12.1786.
3
4
ENZYMATIC EXPRESSION AND GENETIC LINKAGE OF GENES CONTROLLING GALACTOSE UTILIZATION IN SACCHAROMYCES.酿酒酵母中控制半乳糖利用的基因的酶表达与遗传连锁
Genetics. 1964 May;49(5):837-44. doi: 10.1093/genetics/49.5.837.
5
Interference with growth of certain Escherichia coli mutants by galactose.半乳糖对某些大肠杆菌突变体生长的干扰
Biochim Biophys Acta. 1958 Nov;30(2):298-302. doi: 10.1016/0006-3002(58)90054-4.
6
Growth inhibition by alpha-aminoadipate and reversal of the effect by specific amino acid supplements in Saccharomyces cerevisiae.α-氨基己二酸对酿酒酵母的生长抑制作用以及特定氨基酸补充剂对该作用的逆转
J Bacteriol. 1982 Nov;152(2):874-9. doi: 10.1128/jb.152.2.874-879.1982.
7
Two unlinked lysine genes (LYS9 and LYS14) are required for the synthesis of saccharopine reductase in Saccharomyces cerevisiae.酿酒酵母中合成酵母氨酸还原酶需要两个不连锁的赖氨酸基因（LYS9和LYS14）。
J Bacteriol. 1984 Jul;159(1):429-32. doi: 10.1128/jb.159.1.429-432.1984.
8
Ammonia regulation of amino acid permeases in Saccharomyces cerevisiae.酿酒酵母中氨基酸通透酶的氨调节
Mol Cell Biol. 1983 Apr;3(4):672-83. doi: 10.1128/mcb.3.4.672-683.1983.
9
Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae.酿酒酵母中嘧啶生物合成的调控
J Bacteriol. 1968 Mar;95(3):824-32. doi: 10.1128/jb.95.3.824-832.1968.
10
Relationship among the genes, enzymes, and intermediates of the biosynthetic pathway of lysine in Saccharomyces.酿酒酵母中赖氨酸生物合成途径的基因、酶和中间产物之间的关系。
Mol Gen Genet. 1972;115(1):26-30. doi: 10.1007/BF00272214.
Zotero 插件