酿酒酵母中合成酵母氨酸还原酶需要两个不连锁的赖氨酸基因（LYS9和LYS14）。
Two unlinked lysine genes (LYS9 and LYS14) are required for the synthesis of saccharopine reductase in Saccharomyces cerevisiae.
作者信息
Borell C W, Urrestarazu L A, Bhattacharjee J K
出版信息
J Bacteriol. 1984 Jul;159(1):429-32. doi: 10.1128/jb.159.1.429-432.1984.
Abstract
Three lysine auxotrophs, strains AU363, 7305d, and 8201-7A, were investigated genetically and biochemically to determine their gene loci, biochemical lesions, and roles in the lysine biosynthesis of Saccharomyces cerevisiae. These mutants were leaky and blocked after the alpha-aminoadipate step. Complementation studies placed these three mutations into a single, new complementation group, lys14. Tetrad analysis from appropriate crosses provided evidence that the lys14 locus represented a single nuclear gene and that lys14 mutants were genetically distinct from the other mutants (lys1, lys2, lys5, and lys9) blocked after the alpha-aminoadipate step. The lys14 strains, like lys9 mutants, accumulated alpha-aminoadipate-semialdehyde and lacked significant amounts of saccharopine reductase activity. On the bases of these results, it was concluded, therefore, that LYS9 and LYS14, two distinct genes, were required for the biosynthesis of saccharopine reductase in wild-type S. cerevisiae.
摘要
对三种赖氨酸营养缺陷型菌株AU363、7305d和8201 - 7A进行了遗传学和生物化学研究，以确定它们在酿酒酵母赖氨酸生物合成中的基因位点、生化损伤及作用。这些突变体在α - 氨基己二酸步骤之后是渗漏型且受阻的。互补研究将这三个突变归为一个新的互补群，即lys14。来自适当杂交的四分体分析提供了证据，表明lys14位点代表一个单细胞核基因，并且lys14突变体在遗传上与α - 氨基己二酸步骤之后受阻的其他突变体（lys1、lys2、lys5和lys9）不同。与lys9突变体一样，lys14菌株积累α - 氨基己二酸 - 半醛且缺乏大量的酵母氨酸还原酶活性。基于这些结果，因此得出结论，野生型酿酒酵母中酵母氨酸还原酶的生物合成需要LYS9和LYS14这两个不同的基因。
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